@Article{biocell.2017.41.033,
AUTHOR = {SARA SOLTANIAN, HELIA RIAHIRAD, ATHAREH PABARJA, MOHAMMAD REZA KARIMZADEH, KOLSOUM SAEIDI},
TITLE = {Kaempferol and docetaxel diminish side population and down-regulate some cancer stem cell markers in breast cancer cell line MCF-7},
JOURNAL = {BIOCELL},
VOLUME = {41},
YEAR = {2017},
NUMBER = {2-3},
PAGES = {33--40},
URL = {http://www.techscience.com/biocell/v41n2-3/33905},
ISSN = {1667-5746},
ABSTRACT = {Cancer stem cells (CSCs) are a small subpopulation of cancer cells whose resistance to
chemotherapy and radiotherapy plays a pivotal role in treatment failure, tumor recurrence and metastasis.
Today, the ability of many phytochemicals in targeting of CSCs has been identified. Kaempferol is a plant
phytoestrogen that was recognized as a useful agent for targeting cancer cells by apoptosis induction,
cell cycle arrest and inhibition of angiogenesis, migration, and invasion of cancer cells. In this study,
we compared the effect of docetaxel as a common breast cancer chemotherapy drug and kaempferol
on MCF-7 breast CSCs. Results showed that both docetaxel and kaempferol caused a dose-dependent
reduction in cell proliferation. Rhodamin 123 dye staining and flow cytometry analysis revealed that after
docetaxel and kaempferol treatment, SP cells proportion of MCF-7 decreased to 11.83±2.2% and 3±0.2 5%
respectively relative to proportion of untreated control cells (23±3.2%). Moreover, real time PCR results
showed that kaempferol treatment of MCF-7 was more effective than docetaxel in down-regulating the
CSC-associated markers (i.e. oct4, nanog, abcb1 and aldh1a1). Taken together, these results indicate that
kaempferol is an effective anti-CSCs agent, therefore it may be a therapeutic strategy for eradicating breast
cancer through the elimination of CSCs.},
DOI = {10.32604/biocell.2017.41.033}
}