@Article{biocell.2018.07005,
AUTHOR = {Arwa Osama NEMER, Mohammad Saud AL ANAZI, Ramesa Shafi BHAT, Arjumand S. WARSY, Zeneb A BABAY, Mohammad H. ADDAR, Jilani SHAIK, Sooad AL-DAIHAN},
TITLE = {Association between preterm birth risk and polymorphism and expression of the DNA repair genes <i>OGG1</i> and <i>APE1</i> in Saudi women},
JOURNAL = {BIOCELL},
VOLUME = {42},
YEAR = {2018},
NUMBER = {1},
PAGES = {1--6},
URL = {http://www.techscience.com/biocell/v42n1/33521},
ISSN = {1667-5746},
ABSTRACT = {Genomic instability and mutations caused by increases in oxidative stress during pregnancy
can damage the fetoplacental unit and can upshot preterm birth. Oxidative damage to DNA may possibly
be involved in etiology of preterm birth (PTB) which can be repaired by DNA repair gene. In the present
study, we assessed the association of base excision repair gene family by analyzing the association of
single nucleotide polymorphisms and genes expression in 8-oxoguanine <i>glycosylase-1 (OGG1)</i> and
apurinic-apyrimidinic endonuclease 1 (<i>APE1</i>) genes with risk of preterm birth in Saudi women. We
analyzed genotypes of four single nucleotide polymorphisms (SNPs) (rs1052133, rs293795, rs2072668 and
rs2075747) in <i>OGG1</i> gene and three SNPs (rs1130409, rs3136814, and rs3136817) in <i>APE1</i> gene using
TaqMan Genotyping assay kits in 50 pairs of preterm cases and individually matched controls. Also, gene
expression level was explored by RT-PCR in 10 pairs of preterm placental tissues and individually matched
normal placental tissues. Two <i>OGG1</i> SNP, rs1052133 (OR=0.497; c2=1.11; <i>p</i>=0.292) and rs2072668
(OR=0.408; c2=1.90; <i>p</i>=0.167) and one APE1 SNP rs3136817 (OR=0.458; c2=0.40; p=0.527) showed nonsignificant protective effect against PTB development. The expression of both genes under study was
found lower in the PTB patients. Genotype and allele frequencies of both gene SNPs did not show any
association with the risk of preterm delivery in Saudi women (<i>P</i>˃0.05). However, synthesis and release of
<i>OGG1</i> and <i>APE1</i> proteins decreased in preterm placental tissues compared to term delivery reflects the
probability of being one of the mechanisms leading to preterm birth.},
DOI = {10.32604/biocell.2018.07005}
}