@Article{biocell.2019.07019,
AUTHOR = {Hala Abdulaziz M ALWARTHAN, Mohammad Saud AL ANAZI, Narasimha R. PARINE, Ramesa Shafi BHAT, Ghadah ALAMRO, Ftoon ALJARBOU, Sooad K AL-DAIHAN},
TITLE = {The expressional level of tankyrase-1 gene and its regulation in colorectal cancer in a Saudi population},
JOURNAL = {BIOCELL},
VOLUME = {43},
YEAR = {2019},
NUMBER = {2},
PAGES = {51--58},
URL = {http://www.techscience.com/biocell/v43n2/33375},
ISSN = {1667-5746},
ABSTRACT = {Tankyrase1 plays an essential role in cancer progression by regulating telomere length. The study aimed
to determine expression of <i>TNKS1</i> and its regulation in colorectal cancer (CRC) in 20 samples from Saudi patients.
mRNA expression of <i>TNKS1</i> in CRC and paired normal tissues was measured by qRT-PCR. Epigenetic modification
of <i>TNKS1</i> promoter was determined by methylation-specific PCR while somatic mutation was analyzed by Sanger
sequencing in exon 10 of the gene. All cancerous and normal tissues expressed <i>TNKS1</i>, but level of expression in CRC
tissues was significantly associated with tumor stage though no other parameters; age, gender, and tumor location,
showed any correlation. Expression of <i>TNKS1</i> was markedly higher in earlier (I, II) than later (III, IV) stages of CRC
development. Both cancerous and healthy tissues had unmethylated promoter. Sanger sequencing of exon 10 masked
any somatic mutation in the samples. Our findings suggest that up-regulation of <i>TNKS1</i> was inversely correlated with
cancer progression in CRC, indicating that <i>TNKS1</i> participates in the initiation of CRC by stabilizing telomere length in
the first phase of cancer progression. Mechanisms other than <i>TNKS1</i> might play a role in malignant tumor progression
and telomere maintenance in the late stages of CRC.},
DOI = {10.32604/biocell.2019.07019}
}