@Article{biocell.2019.06231,
AUTHOR = {Mahmoud MOUSTAFA, Saad ALAMRI, Hoida ZAKI, Naglaa LOUTFY, Tarek TAHA, Ali SHATI, Mohamed ALKAHTANI, Sajda SIDDIQI},
TITLE = {Gene expression of 49 kDa apyrase, cytoskeletal proteins, ATPase, ADPase and amino acid contents of <i>Pisum sativum</i> (L.) cells germinated in E<i>uryops arabicus</i> (Steud. ex Jaub. \& Spach) water extract},
JOURNAL = {BIOCELL},
VOLUME = {43},
YEAR = {2019},
NUMBER = {2},
PAGES = {81--88},
URL = {http://www.techscience.com/biocell/v43n2/33384},
ISSN = {1667-5746},
ABSTRACT = { The present research reports of quick and marked changes induced by plant extract of <i>Euryops arabicus</i> in the
gene expression of 49-kDa apyrases, cytoskeletal proteins, ATPases, ADPase and amount of amino acid of pea (<i>Pisum
sativum</i> L. var. Alaska). Pellets of cytoskeletals proteins (27000 xg) were probed with anti-apyrase antibody, biotinylated
anti-rat, actin and alpha and beta-tubulin for Western blotting. ATPase and ADPase activities were determined based
on the hydrolytic efficacy of adenine triphosphate and adenine diphosphate. By 72 hours, the abundance of apyrases,
cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds in E. <i>arabicus</i> extracts
were sharply increased than those sown in distilled water. All the samples exhibited that the stems had more amount
from apyrases, cytoskeletal proteins, amino acids and ATPase and ADPase activities than primary leaves and primary
roots that were germinated either on E. <i>arabicus</i> water extract or in distilled water. Based on the enzyme’s capability to
hydrolyse nucleotide triphosphate and nucleotide diphosphate as well as the direct association between expression of
49-kDa apyrase and cytoskeletal proteins, E. <i>arabicus</i> water extract had an important effect on plant germinations.},
DOI = {10.32604/biocell.2019.06231}
}