@Article{biocell.2019.06814,
AUTHOR = {Mahmoud MOUSTAFA, Saad ALAMRI, Tarek TAHA, Ali SHATI, Sulaiman ALRUMMAN, Mohamed ALKAHTANI},
TITLE = {Cloning and characterization of 66 kDa streptavidin-binding peptides (SBP) of <i>Pisum sativum</i> L. embryo specific to var. Alaska},
JOURNAL = {BIOCELL},
VOLUME = {43},
YEAR = {2019},
NUMBER = {3},
PAGES = {155--166},
URL = {http://www.techscience.com/biocell/v43n3/33415},
ISSN = {1667-5746},
ABSTRACT = { The aim of the current research was to clone and to characterize the partial 66 kDa streptavidin-binding
peptide (SBP) found in the germinated embryos of Pisum sativum L. var. Alaska. The pea (<i>P. sativum</i> var. Alaska)
embryos possess prominent 66 kDa SBPs that gradually disappeared after few hours of germination in germinated
embryos, but not in the cotyledons. The total RNA was isolated from embryos of <i>P. sativum</i> but could not be isolated
from the cotyledons. The partial nucleotides sequences of 66 kDa SBPs of embryonic stalk (<i>P. sativum</i> var. Alaska) were
cloned and identified using <i>pMOSBlue</i> vector. 66 kDa (SBP) gene from the embryos of <i>P. sativum</i> var. Alaska possesses
327 bp having an open reading frame (ORF) region in a part of the gene that encoded for 108 amino acids. Alignment
showed similarity among 66 kDa SBPs <i>P. sativum</i> var. Alaska, with <i>P. sativum</i> seed biotinylated protein (SBP65) and <i>P. sativum</i> sbp65a mRNA with DNA distance matrix between 0.0094 to 1.2676. MALDI-TOF mass spectrometry analysis
of 66 kDa (SBP) proteins showed it had similar short peptides to 19 proteins found in different organisms, especially <i>Convicilin precursor</i>, and the seed biotinylated protein in <i>P. sativum</i>. The alignment results of both nucleotide sequences
and amino acid residues either from cloning or MALDI-TOF-MS showed differences with related species, especially <i>P. sativum</i>. No mRNA was found in the cotyledons during seeds germination, which means no metabolic activities and
this part may act only as food reservoirs for growing newly embryos.},
DOI = {10.32604/biocell.2019.06814}
}