TY  - EJOU
AU  - Kamil, Atif 
AU  - Khan, Mubarak Ali 
AU  - AAsim, Muhammad 
AU  - Khan, Nadir Zaman 
AU  - Khan, Raham Sher 
AU  - Jamal, Muhsin 
AU  - Ahmad, Waqar 
AU  - Khan, Mir Azam 
AU  - Jalil, Fazal 

TI  - Detection of ROS and translocation of ERP-57 in apoptotic induced human neuroblastoma (SH-SY5Y) cells
T2  - BIOCELL

PY  - 2019
VL  - 43
IS  - 3
SN  - 1667-5746

AB  -  Several toxic compounds are known to induce apoptosis in mammalian cell lines. The human neuroblastoma
cells (SH-SY5Y) were exposed to the phosphatase inhibiting toxin okadaic acid (OA) or hydrogen peroxide (H2O2)
to induce apoptosis as well as generate reactive oxygen species (ROS). Mitoxantrone (MXT) was used as a positive
control for apoptosis. The SH-SY5Y cells were transfected with eukaryotic expression plasmid pHyPer-dMito encoding
mitochondrial-targeted fluorescent or pHyPer-dCito encoding cytoplasmic-targeted fluorescent sensor for hydrogen
peroxide (HyPer). The ERp57, also called GRP58 (Glucose-regulated protein 58), is a stress protein induced in
conditions like glucose starvation and viral infection. Recently ERp57 was shown to translocate from the endoplasmatic
reticulum to the cell surface in anthracycline-induced apoptotic cells. ERp57 co-translocation together with calreticulin
has been suggested to be crucial for recognizing tumor cells to induce immunogenic cell death. ERp57 translocation
after exposure to okadaic acid was studied using immunofluorescence and confocal microscopy. These studies indicated
that okadaic acid has induced the translocation of ERp57 to the cellular membrane.
KW  - Apoptosis
KW  -  Cytoplasm
KW  -  Endoplasmic recticulum (ER)
KW  -  ERP-57
KW  -  Human neuroblastoma cell (SH-SY5Y)
KW  -  Immunofluorescence
KW  -  Mitochondria
KW  -  Reactive oxygen species (ROS)
KW  -  Transfection

DO  - 10.32604/biocell.2019.06729