@Article{biocell.2019.07992,
AUTHOR = {Heya QIAN, Yan YAN, Zhengjie SHEN, Lixian XU, Yun ZUO, Tao ZHU, Yanan CHEN},
TITLE = {p53 siRNA promotes autophagy of U2OS cells through its target gene Rap2B},
JOURNAL = {BIOCELL},
VOLUME = {43},
YEAR = {2019},
NUMBER = {4},
PAGES = {321--326},
URL = {http://www.techscience.com/biocell/v43n4/38132},
ISSN = {1667-5746},
ABSTRACT = {The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS
cells. U2OS cells were treated with siRNA against p53, Rap2B, and PLCε. Relative expressions of p53, Rap2B, and PLCε
were determined using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Levels of IP3
in the cells were determined using Enzyme-linked Immunosorbent Assay (ELISA). Levels of Ca<sup>2+</sup> were detected using Flow cytometry. Fluorescence microscopy was used to observe the autophagy of cells. Knockdown of p53 significantly
decreased the expressions of Rap2B protein. Additionally, knockdown of p53 significantly decreased the mRNA levels
of PLCε. The knockdown of p53, Rap2B, and PLCε significantly decreased the levels of intracellular IP3 and Ca<sup>2+</sup> and promoted autophagy of U2OS cells. Our results demonstrated that p53-Rap2B-PLCε-IP3 signaling pathway regulated
autophagy of U2OS cells.},
DOI = {10.32604/biocell.2019.07992}
}