@Article{biocell.2020.08324,
AUTHOR = {Xin YANG, Liqun LU, Li HUANG, Jing HE, Jie LV},
TITLE = {MicroRNA-145-3p suppresses the malignant behaviors of T-cell acute lymphoblastic leukemia Jurkat cells via inhibiting the NFkappaB signaling pathway},
JOURNAL = {BIOCELL},
VOLUME = {44},
YEAR = {2020},
NUMBER = {1},
PAGES = {101--110},
URL = {http://www.techscience.com/biocell/v44n1/38429},
ISSN = {1667-5746},
ABSTRACT = {T-cell acute lymphoblastic leukemia (T-ALL) is a hematological tumor caused by the malignant
transformation of immature T-cell progenitor cells. Emerging studies have stated that microRNAs (miRNAs) may play
key roles in T-ALL progression. This study aimed to investigate the roles of miR-145-3p in T-ALL cell proliferation,
invasion, and apoptosis with the involvement of the nuclear factor-kappaB (NF-κB) signaling pathway. T-ALL Jurkat
cells were harvested, and the expression of miR-145-3p and NF-κB-p65 was measured. Gain- and loss-of-functions of
miR-145-3p and NF-κB-p65 were performed to identify their roles in the biological behaviors of Jurkat cells, including
proliferation, apoptosis, and invasion. Consequently, the current study demonstrated that miR-145-3p was downregulated
while NF-κB-p65 was up-regulated in Jurkat cells. miR-145-3p directly bound to the 3’ untranslated region
of NF-κB-p65. Over-expression of miR-145-3p inhibited Jurkat cell proliferation, invasion, and resistance to apoptosis,
while over-expression of NF-κB-p65 presented opposite trends. Co-transfection of miR-145-3p and NF-κB-p65
promoted the malignant behaviors of Jurkat cells compared to miR-145-3p transfection alone, while it reduced these
behaviors of Jurkat cells compared to NF-κB-p65 transfection alone. Taken together, this study provided evidence that
miR-145-3p could suppress proliferation, invasion, and resistance to the death of T-ALL cells via inactivating the NF-
κB signaling pathway.},
DOI = {10.32604/biocell.2020.08324}
}