TY  - EJOU
AU  - YANG, Xin 
AU  - LV, Liqun LU, Li HUANG, Jing HE, Jie 

TI  - MicroRNA-145-3p suppresses the malignant behaviors of T-cell acute lymphoblastic leukemia Jurkat cells via inhibiting the NFkappaB signaling pathway
T2  - BIOCELL

PY  - 2020
VL  - 44
IS  - 1
SN  - 1667-5746

AB  - T-cell acute lymphoblastic leukemia (T-ALL) is a hematological tumor caused by the malignant
transformation of immature T-cell progenitor cells. Emerging studies have stated that microRNAs (miRNAs) may play
key roles in T-ALL progression. This study aimed to investigate the roles of miR-145-3p in T-ALL cell proliferation,
invasion, and apoptosis with the involvement of the nuclear factor-kappaB (NF-κB) signaling pathway. T-ALL Jurkat
cells were harvested, and the expression of miR-145-3p and NF-κB-p65 was measured. Gain- and loss-of-functions of
miR-145-3p and NF-κB-p65 were performed to identify their roles in the biological behaviors of Jurkat cells, including
proliferation, apoptosis, and invasion. Consequently, the current study demonstrated that miR-145-3p was downregulated
while NF-κB-p65 was up-regulated in Jurkat cells. miR-145-3p directly bound to the 3’ untranslated region
of NF-κB-p65. Over-expression of miR-145-3p inhibited Jurkat cell proliferation, invasion, and resistance to apoptosis,
while over-expression of NF-κB-p65 presented opposite trends. Co-transfection of miR-145-3p and NF-κB-p65
promoted the malignant behaviors of Jurkat cells compared to miR-145-3p transfection alone, while it reduced these
behaviors of Jurkat cells compared to NF-κB-p65 transfection alone. Taken together, this study provided evidence that
miR-145-3p could suppress proliferation, invasion, and resistance to the death of T-ALL cells via inactivating the NF-
κB signaling pathway.
KW  - T-cell acute lymphoblastic leukemia
KW  -  Proliferation
KW  -  Invasion
KW  -  Resistance to apoptosis

DO  - 10.32604/biocell.2020.08324