@Article{biocell.2020.09097,
AUTHOR = {SAIDA MELIANI, SOUMAYA TOUMI, HEYTHEM DJAHOUDI, KHALED DEGHDEGH, KAMEL AMOURA, ABDELGHANI DJAHOUDI},
TITLE = {Synergistic combination of colistin with imipenem, amikacine or ciprofloxacin against <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i> carbapenem-resistant isolated in Annaba hospital Algeria},
JOURNAL = {BIOCELL},
VOLUME = {44},
YEAR = {2020},
NUMBER = {2},
PAGES = {175--182},
URL = {http://www.techscience.com/biocell/v44n2/39268},
ISSN = {1667-5746},
ABSTRACT = {<b>Objective:</b> The aim of this study is to detect in vitro the synergetic activity of colistin in combination with
imipenem, amikacin or ciprofloxacin, at sub-inhibitory concentrations, against carbapenems-resistant (CR) <i>Acinetobacter
baumannii</i> and <i>Pseudomonas aeruginosa</i> strains isolated from various wards in Annaba teaching hospital in eastern Algeria.<br/>
<b>Materials and Methods:</b> The minimal inhibitory concentrations (MIC) were determined by broth macrodilution (BMD).
Carbapenemase encoding genes were screened using polymerase chain reaction (PCR). The activity of colistin in
combination with second antibiotic was evaluated by the Checkerboard Technique.<br/>
<b>Results:</b> 39 CR <i>P. aeruginosa</i> and 21 CR <i>A. baumanni</i> strains where collected. The MIC values ranging from (0.25 to
4 µg/ml) to colistin, ≥16 µg/ml for imipenem, ≥4 µg/ml to amikacin and ≥8 µg/ml ciprofloxacin. The PCR reveals the
presence of the genes bla<sub>OXA23</sub> (n = 12), bla<i>OXA24</i> (n = 6), bla<i>NDM1</i> (n = 3) in <i>A. baumannii</i> and bla<i>VIM2</i> (n = 12) in <i>P.
aeruginosa</i>. The combination of colistin with imipenem showed synergistic effect on 57.14% and 46.15% of <i>A.
baumannii</i> and <i>P. aeruginosa</i> isolates, respectively. For colistin and amikacin, the synergistic effect is detected in 28.6%
of <i>A. baumannii</i> and 30.8% of <i>P. aeruginosa</i>. While colistin and ciprofloxacin showed synergy on 14.29% and 15.38% of
<i>A. baumannii</i> and <i>P. aeruginosa</i> isolates, respectively.<br/>
<b>Conclusion:</b> CR <i>A. baumannii</i> and <i>P. aeruginosa</i> remain the most prevalent infection agents in patients from high-risk
wards at Annaba Hospital. Colistin associated with imipenem or with amikacin at sub-inhibitory concentrations gives
very encouraging results allowing better management of infections caused by this type of bacteria.},
DOI = {10.32604/biocell.2020.09097}
}