@Article{biocell.2020.09051,
AUTHOR = {HAIFENG WANG, JUAN GUO, LIJING ZHENG, SHUYING LIU, ZHERONG WANG, HONGXUAN HE},
TITLE = {Detection of new antibiotic resistance gene profile in <i>Escherichia coli</i> associated with avian leukosis virus infection from broiler chickens},
JOURNAL = {BIOCELL},
VOLUME = {44},
YEAR = {2020},
NUMBER = {2},
PAGES = {217--224},
URL = {http://www.techscience.com/biocell/v44n2/39273},
ISSN = {1667-5746},
ABSTRACT = {The <i>Escherichia coli</i> (<i>E. coli</i>) is prevailing worldwide, but the epidemiology of <i>E. coli</i> infections feature regional
distribution characteristics to some extent. <i>E. coli</i>, as a zoonotic pathogen, can be transferred from animals to humans
through food chain or via contact with wounds, causing a public health risk. We reported the swelling of
proventriculus and tracheal bleeding following the death in two broiler chickens (<i>Gallus gallus domesticus</i>) from
Beijing, China. To investigate whether a virus was involved in the infection, Madin Darby Bovine Kidney (MDCK)
cells were co-cultured with supernatants of proventriculus, trachea and spleen homogenates. The avian leucosis virus
was detected in the samples of proventriculus and trachea, but the avian influenza virus, the Newcastle disease virus
and the avian infectious laryngotracheitis virus were not detected. <i>E. coli</i> isolates were resistant to almost all the
antimicrobial as tested except for the combinations of amoxicillin/clavulanic acid and sulfamethoxazole/trimethoprim.
PCR tests demonstrated the presence of antibiotic resistance genes in these <i>E. coli isolates</i> and further research
revealed a novel gene profile with the presence of CTX-M-1, gyrA, gyrB, oqxA, oqxB, parC and Sul2 antibiotic
resistance genes in a strain isolated from a proventriculus sample. These results demonstrated that the presence of
antibiotic resistant <i>E. coli</i> would not necessarily cause outbreak of large-scale disease. However, when the bacteria
carrying new antibiotic resistance genes enter the environment, it may result in the development of more virulent
strains which will potentially impact human and animal health.},
DOI = {10.32604/biocell.2020.09051}
}