@Article{biocell.2020.012342,
AUTHOR = {MARÍA ELISA MILLÁN, MARÍA FERNANDA MARRA, LEONARDO ANDRÉS SALVARREDI, EMILIO FERNANDO LIZARRAGA, LUIS ALBERTO LOPEZ},
TITLE = {Fractions of a chloroform extract of ajenjo leaves (<i>Artemisia mendozana</i> DC. var. <i>mendozana</i>) inhibit the proliferation, viability and clonogenicity of B16-F0 melanoma cells},
JOURNAL = {BIOCELL},
VOLUME = {44},
YEAR = {2020},
NUMBER = {3},
PAGES = {293--299},
URL = {http://www.techscience.com/biocell/v44n3/40228},
ISSN = {1667-5746},
ABSTRACT = {The ajenjo, <i>Artemisia mendozana</i> DC. var. <i>mendozana</i> (Asteraceae), grows in the Andean foothills of Mendoza
and San Juan, Argentina, and is used as a medicinal plant for its antispasmodic and antifungal properties. The aim of this
work was to obtain fractions of a chloroform extract of ajenjo leaves and to evaluate the <i>in vitro</i> effects on proliferation,
viability and clonogenicity of B16-F0 melanoma cells. Using a silica gel chromatography column, 120 fractions were
collected and grouped according to the chromatographic profile in 9 main fractions (F1–F9). Their major compounds
identified were: terpenes (F1), terpenes and sesquiterpene lactones (F2–F3), sesquiterpenes (F4–F6) and phenols and
sesquiterpenes (F7-9). B16-F0 cells were incubated for 72 h with DMSO (vehicle) or 0.1 mg/ml F1–F9. At 72 h of
culture, F1 decreased both the growing index (GI) and cell viability. F2 and F3 both decreased GI and only F3
decreased clonogenic activity. F4 and F5 both decreased GI. Only F5 decreased cell viability and F4 decreased
clonogenicity. Consequently, fractions F6–F8 did not affect any of the cell parameters assayed, while F9 decreased cell
viability and inhibited clonogenicity.},
DOI = {10.32604/biocell.2020.012342}
}