@Article{biocell.2020.09170,
AUTHOR = {YUNZE XUAN, BIN JIN, SAYAN DEB DUTTA, MENGMENG LIU, ZAIXIAN SHEN, XIWEN LIU, YANG KANGJUAN, LIM KI-TAEK},
TITLE = {Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid},
JOURNAL = {BIOCELL},
VOLUME = {44},
YEAR = {2020},
NUMBER = {3},
PAGES = {389--400},
URL = {http://www.techscience.com/biocell/v44n3/40239},
ISSN = {1667-5746},
ABSTRACT = {Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the
surface of the teeth, leading to the destruction of tooth-supporting tissues and loss of alveolar bone. Suberoylanilide
hydroxamic acid (SAHA), a class of histone deacetylase (HDAC) inhibitor, has the potential to stimulate osteoblast
differentiation by acetylating histone proteins, and thus suppressing the expression of adipogenic and chondrogenic
genes. However, the effect of SAHA on the differentiation of human periodontal ligament stem cells (hPDLSCs) is yet
to be elucidated. Herein, we investigated the effects of SAHA on in vitro proliferation and differentiation of hPDLSCs
by MTT assay, Alizarin Red-S, and alkaline phosphatase staining, and real-time PCR. Notably, 300 ng/mL SAHA
treatment enhanced the proliferation and mineralization of hPDLSCs, indicating their osteogenic potential. Moreover,
a significant enhancement of osteogenesis gene markers and proteins was observed. We also demonstrated that ERK
is a positive regulator of Runx2 transcription factors during osteoblast differentiation. These results indicate that
SAHA may be a useful osteogenic induction agent for periodontal bone regeneration.},
DOI = {10.32604/biocell.2020.09170}
}