@Article{biocell.2020.08976,
AUTHOR = {SOHEILA HASANIAN, NOUR AMIRMOZAFARI, AHMAD FARAJZADEH SHEIKH, IRAJ MEHREGAN},
TITLE = {Molecular typing of methicillin and vancomycin-resistant <i>Staphylococcus aureus</i> isolated from clinical specimens by doublelocus sequence typing (DLST) method},
JOURNAL = {BIOCELL},
VOLUME = {44},
YEAR = {2020},
NUMBER = {3},
PAGES = {411--419},
URL = {http://www.techscience.com/biocell/v44n3/40241},
ISSN = {1667-5746},
ABSTRACT = {Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) strains are the essential cause of infections in communities and
hospitals. The present study was conducted to determine the molecular typing of MRSA, isolated from hospitalized patients,
using the double-locus sequence typing (DLST). In total, 280 <i>S</i>. aureus isolated from clinical specimens by phenotypic
(catalase, coagulase, DNase, oxacillin, vancomycin screening agar and antibiotic disk diffusion), and molecular methods
(PCR for determining the <i>mecA</i>, <i>vanA</i> and <i>nuc</i> genes). The DLST and sequencing was performed for MRSA containing
<i>mecA</i>. Out of 280 specimens, confirmed as Staphylococcus aureus (S. aureus), 123 (43.9%) strains were MRSA. The
highest resistance toward the erythromycin (15 μg), followed by ciprofloxacin (5 μg), clindamycin (2 μg), tetracycline (30
μg), gentamicin (10 μg) and rifampicin (5 μg), was 98.3%, 97.5%, 94.3%, 90.2%, 83.7% and 41.4%, respectively. Also, the
least resistance (0%) was observed in each of teicoplanin (30 μg), linzolide (30 μg), and vancomycin (30 μg). All (100%)
of MRSA strains had the <i>mecA</i>, and none of them have had the vanA. The results of DLST showed that the most
common sequence types were BPH 2003 and 0217. The DLST type 18-32 was a significant cluster of MRSA. By
sequencing MRSA and comparing the dominant types via the DLST, it is possible to establish the etiology of the disease
in a much shorter time, and prevent the complications of the disease. Therefore, the combination of partial sequences of
<i>clfB</i> and <i>spa</i> can serve as useful genetic markers for MRSA typing. It concluded that the MRSA in our region was
relatively high, but no vancomycin resistance was found. The majority of the MRSA DLST type was 18–32.},
DOI = {10.32604/biocell.2020.08976}
}