@Article{biocell.2020.010575,
AUTHOR = {ZHIHUI WANG, YE YUAN},
TITLE = {Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from <i>Phragmites australis</i>},
JOURNAL = {BIOCELL},
VOLUME = {44},
YEAR = {2020},
NUMBER = {4},
PAGES = {731--736},
URL = {http://www.techscience.com/biocell/v44n4/40985},
ISSN = {1667-5746},
ABSTRACT = {Glyphosate is a non-selective broad-spectrum herbicide that blocks plant growth by inhibiting 5-
Enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway in microorganisms and
plants. The full-length <i>epsps</i> cDNA sequence (<i>paepsps</i>, Genebank: KY860582.1) was cloned and characterized for the
first time from <i>Phragmites australis</i>. The full-length cDNA of <i>paepsps</i> was 1308 bp encoding a polypeptide of 435
amino acids. The bioinformatic analyses showed that PaEPSPS has highly homologous with EPSPS from other plants.
RT-PCR analysis of <i>paepsps</i> expression indicated that the gene expressed in leaves, stems, and roots, with higher
expression in leaves. The expression of the <i>paepsps</i> gene increased with glyphosate application. In addition, the
transgenic tobacco containing the <i>paepsps</i> gene showed glyphosate resistance in comparison with control. The novel
<i>paepsps</i> is a good candidate gene in transgenic crops with glyphosate tolerance in the future.},
DOI = {10.32604/biocell.2020.010575}
}