@Article{biocell.2020.010207,
AUTHOR = {ALI RAZA, ABU BAKR UMER FAROOQ, WAQAR AHMAD KHAN, AHSAN IQBAL, SADETTIN ÇELİK, MAHWISH ALI, RAO SOHAIL AHMAD KHAN},
TITLE = {Polymorphic information and genetic diversity in <i>Brassica</i> species revealed by RAPD markers},
JOURNAL = {BIOCELL},
VOLUME = {44},
YEAR = {2020},
NUMBER = {4},
PAGES = {769--776},
URL = {http://www.techscience.com/biocell/v44n4/40992},
ISSN = {1667-5746},
ABSTRACT = {Randomly amplified polymorphic DNA (RAPD) is a tremendously convenient approach used to discriminate
between <i>Brassica</i> species owing to its accuracy and speed. RAPD primers generate adequate genetic information that can
be used in the primer-marker system. In this work, twenty RAPD-PCR based markers were executed to generate
polymorphic data, like polymorphic information content (PIC), mean resolving power (MRP), resolving power (RP),
effective multiplex ratio (EMR), and marker index (MI) for the first time and genetic distance among and between six
Brassica species were calculated. Our results indicated that 20 primers produced a total of 231 scored band and
generated 87% polymorphic bands. Average PIC, MRP, RP, MI, and EMR values were 0.088, 0.65, 6.7, 0.78, and 8.9,
respectively. PIC showed an overall negative correlation with MRP, RP, MI, and EMR, whereas MRP, RP, and EMR,
were positively correlated with each other. Genetic identities ranged from 41.99% (between <i>Brassica napus</i> and
<i>Brassica oleracea</i>) to 68.83% (between <i>Brassica campestris</i> and <i>Brassica oleracea</i>). Dendrogram results showed no
clustering between species except between <i>Brassica campestris</i> and <i>Brassica nigra</i>. Nevertheless, these results will be
helpful to acquire useful information about the markers and their use to determine the genomic structures of <i>Brassica</i>
species. Further, based on genetic distance and polymorphic information, new hybrids can be developed for effective
oilseed production.},
DOI = {10.32604/biocell.2020.010207}
}