TY  - EJOU
AU  - SHEN, HANYU 
AU  - REN, SHIQI 
AU  - WANG, WEI 
AU  - ZHANG, CHENLIN 
AU  - HAO, HAIYAN 
AU  - SHEN, QIUYAN 
AU  - DUAN, YINONG 
AU  - WANG, ZIHENG 
AU  - GE, WENLIANG 

TI  - Profiles of immune status and related pathways in sepsis: evidence based on GEO and bioinformatics
T2  - BIOCELL

PY  - 2020
VL  - 44
IS  - 4
SN  - 1667-5746

AB  - Sepsis, characterized as life-threatening sequential organ failure, is caused by a dysregulated host immune
response to a pathogen. Conventional practice for sepsis is to control the inflammation source and administer highgrade antibiotics. However, the mortality rate of sepsis varies from 25–30% and can reach 50% if a septic shock
occurs. In our current study, we used bioinformatics technology to detect immune status profiles in sepsis at the
genomic level. We downloaded and analyzed gene expression profiles of GSE28750 from the Gene Expression
Omnibus (GEO) database to determine differential gene expression and immune status between sepsis and normal
samples. Next, we used the CIBERSORT method to quantify the proportions of immune cells in the sepsis samples.
Then we explored the differentially expressed genes (DEGs) related to sepsis. Furthermore, gene ontology (GO)
function and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to
present potential signaling pathways in sepsis. We found that in the sepsis samples, the CD8<sup>+</sup> T cell fraction was
consistently lower, based on the CIBERSORT method, whereas the neutrophil fraction was significantly higher in the
sepsis samples. The GO function and KEGG pathway enrichment analysis identified 1573 DEGs that were
significantly associated with neutrophil activation, neutrophil degranulation, neutrophil activation involved in the
immune response, neutrophil-mediated immunity, and T cell activation in the biological processes group. In our
study, we provided a first glance of associations between immune status and sepsis. Furthermore, our data regarding
the reciprocal interaction between immune cells (neutrophils and CD8<sup>+</sup> T cells) could improve our understanding of
immune status profiles in sepsis. However, additional investigations should be performed to verify their clinical value.
KW  - Sepsis
KW  -  GEO
KW  -  Bioinformatics
KW  -  Neutrophil
KW  -  CD8<sup>+</sup> T cell

DO  - 10.32604/biocell.2020.011345