@Article{biocell.2020.011713,
AUTHOR = {ZICHEN LIU, SIYAO GUO, MENGZHI JI, KAILI SUN, ZHONGFANG LI, XIANGYU FAN},
TITLE = {Progresses of mycobacteriophage-based <i>Mycobacterium tuberculosis</i> detection},
JOURNAL = {BIOCELL},
VOLUME = {44},
YEAR = {2020},
NUMBER = {4},
PAGES = {683--694},
URL = {http://www.techscience.com/biocell/v44n4/40999},
ISSN = {1667-5746},
ABSTRACT = {Tuberculosis (TB) remains a major cause of morbidity and mortality worldwide, particularly in developing
countries. A rapid and efficient method for TB diagnosis is indispensable to check the trend of tuberculosis
expansion. The emergence of drug-resistant bacteria has increased the challenge of rapid drug resistance tests. Due to
its high specificity and sensitivity, bacteriophage-based diagnosis is intensively pursued. In this review, we mainly
described mycobacteriophage-based diagnosis in TB detection, especially two prevalent approaches: fluorescent
reporter phage and phage amplified biologically assay (PhaB). The rationale of reporter phage is that phage carrying
fluorescent genes can infect host bacteria specifically. Phage amplified biological assay based on the principle that
phages can infect the live <i>Mycobacterium tuberculosis</i>< in the specimen under suitable conditions and produce plaques.
Other phage-based diagnostic methods, such as a combination of the amplified biologically assay and nucleic acid
amplification or lateral flow assays, are also actively explored. This review will help us improve the understanding of
mycobacteriophages in TB detection and better promote the development of the rapid diagnosis of <i>M. tuberculosis</i>.},
DOI = {10.32604/biocell.2020.011713}
}