@Article{biocell.2020.012645,
AUTHOR = {QI SONG, JUN ZHANG, QIANG ZHANG, JING LIU, KE LV, JIALU YAO, YAFENG ZHOU},
TITLE = {Exosomes derived from circBCRC-3-knockdown mesenchymal stem cells promoted macrophage polarization},
JOURNAL = {BIOCELL},
VOLUME = {44},
YEAR = {2020},
NUMBER = {4},
PAGES = {623--629},
URL = {http://www.techscience.com/biocell/v44n4/41002},
ISSN = {1667-5746},
ABSTRACT = {Macrophages play an essential role in the myocardial ischemia-reperfusion injury (MIRI), and the macrophage
shifting from M1 to M2 phenotypes might be a potential strategy for the treatment of MIRI. It has been reported that
miR-182 plays an important role in MSC-Exo-associated macrophage polarization. As circBCRC-3 is a newly
discovered circle RNA that worked as a sponge of miR-182, this research aimed to find if circBCRC-3 plays a role in
MSC-Exo-associated macrophage polarization. Firstly, circBCRC-3 was identified by divergent primers in
mesenchymal stem cells (MSCs). Secondly, the exosome of MSCs was isolated and identified by transmission electron
microscopy (TEM), nanoparticle-tracking analysis, and western blotting analysis. The expression level of circBCRC-3
in MSCexos was detected by RT-PCR. Finally, the polarization of the RAW264.7 cell phenotype was analyzed by flow
cytometry. Moreover, we first identified circBCRC-3 in MSCs. The results further confirmed that MSCexo could
effectively shift the macrophage polarization state from M1 towards the M2 phenotype, which indicated its role
in MIRI cure.},
DOI = {10.32604/biocell.2020.012645}
}