@Article{biocell.2021.011756,
AUTHOR = {YANGUO PENG, WEI LI, SHUIYU CHEN, WANGCHUN WU, ZHANFANG SHANG},
TITLE = {MiR-494-3p regulates cell proliferation and apoptosis via KLF7 in Schwann cells},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {1},
PAGES = {57--64},
URL = {http://www.techscience.com/biocell/v45n1/41400},
ISSN = {1667-5746},
ABSTRACT = {Peripheral nerve injury is a common neurodegenerative disease, which causes disability and a huge economic
burden for patients. MicroRNAs (miRNAs) have been acknowledged as major regulators and therapeutic targets of
neurological disease. Thus, the functional studies of miRNAs in neurological disease will contribute to discover new
therapeutic targets for peripheral nerve injury. Sprague Dawley rats treated sciatic nerve surgical injury were regarded
as peripheral nerve injury model in vivo. The expression of miR-494-3p and Kruppel like factor7 (KLF7) were
measured by Real-time quantitative polymerase chain reaction (RT-qPCR) assay. In addition, western blot analysis
was conducted to measure the protein levels of KLF7, Bax, Bcl-2, and C-caspase 3. Cell viability and apoptosis were
detected in Schwann cells by EdU stain and flow cytometry, respectively. The interaction between miR-494-3p and
KLF7 was investigated by dual-luciferase reporter assay. The expression of miR-494-3p was reduced at the beginning,
but KLF7 was enhanced in Sprague Dawley rats with peripheral nerve injury. Knockdown of miR-494-3p promoted
cell proliferation and suppressed apoptosis, while overexpression of miR-494-3p or silencing KLF7 led to opposite
results. Moreover, the upregulation of KLF7 attenuated miR-494-3p overexpression-induced suppressive effects on
viability and promotion of apoptosis in Schwann cells. MiR-494-3p negatively regulates KLF7 in Schwann cells to
mediate proliferation and apoptosis.},
DOI = {10.32604/biocell.2021.011756}
}