@Article{biocell.2021.013583,
AUTHOR = {YONG LIU, ZHIHUA JU, QIANG JIANG, WENHAO LIU, CHUNHONG YANG, YARAN ZHANG, XIUGE WANG, YAPING GAO, XIAOCHAO WEI, YAN SUN, JINPENG WANG, MINGHAI HOU, LING YANG, JINMING HUANG},
TITLE = {DNA methylation and alternative splicing modulate FBXW11 gene expression in Holstein bull testis and are correlated with sperm quality},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {1},
PAGES = {79--87},
URL = {http://www.techscience.com/biocell/v45n1/41402},
ISSN = {1667-5746},
ABSTRACT = {F-box and WD-40 domain protein 11 (FBXW11) is an important component of the E3 ubiquitin-ligase enzyme
that plays a key role in the ubiquitin-dependent regulation of spermatogenesis. In our previous research, the mRNA
expression of FBXW11 in bull sperm with high motility is significantly higher than that with low motility. In the
present study, the protein expression levels of FBXW11 in bull testicular tissues with low-performance sperm quality
groups were significantly higher than those in normal performance groups. The immunohistochemistry result
demonstrated that FBXW11 protein was located in the periphery of Leydig cells and seminiferous tubules. Three
splice variants of the FBXW11 gene, namely, FBXW11-tv1, FBXW11-tv2, and FBXW11-tv3, were identified in
testicular tissues. The splicing patterns of the three variants are exon skipping. The transcript FBXW11-tv2
expressions were the highest in each sample. The low-performance groups displayed higher FBXW11-tv1 and
FBXW11-tv2 transcript expressions than the normal performance groups. Two CpG islands were located within the 5’
UTR and exon 1-2 region of the FBXW11 gene. Bisulfite sequencing PCR results demonstrated that the methylation
levels of 11 methylation sites in the CpG island 2 from −99 to −43 in the normal performance groups were
significantly lower than those in the low-performance groups. Pearson correlation analysis suggested that the CpG
island 2 methylation level was negatively correlated with sperm motility and the transcript FBXW11-tv2 expression
level. Our data revealed that alternative splicing and DNA methylation jointly regulated FBXW11 gene expression and
were correlated with sperm quality traits during spermatogenesis in Holsteins.},
DOI = {10.32604/biocell.2021.013583}
}