@Article{biocell.2021.012011,
AUTHOR = {SALLY NEGM, MOHAMMAD EL-METWALLY, WESAM ELDIN SABER, SAHAR ABO-NEIMA, MAHMOUD MOUSTAFA, ATTALLA EL-KOTT},
TITLE = {Nanoparticles induce the biosynthesis and activity of the new possible therapeutic proteinase source, <i>Talaromyces purpureogenus</i> KJ584844},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {1},
PAGES = {119--127},
URL = {http://www.techscience.com/biocell/v45n1/41406},
ISSN = {1667-5746},
ABSTRACT = {The need for the bacterial proteinase is rapidly growing, urging to catch a lowcost medium for the microbial
fermentation, nanoparticles can play a vital role in this respect. The proteinase of <i>Talaromyces purpureogenus</i> was
produced on the tubers of <i>Helianthus tuberosus</i> that also operated as solid support for the fermentation process. The
interface amongst nitrogen sources (NH<sub>4</sub>Cl and yeast extract) was investigated, applying the statistical modeling of
central composite design under solid-state fermentation. The optimum medium for proteinase secretion was
stimulated by 979.82 mg NH<sub>4</sub>Cl and 437.68 mg yeast extract per 100 g substrate, yielding 108.15 U/g tubers. Using
Plackett-Burman experimental design, the nanoparticles Co, Ni and Fe were assessed as inducers for proteinase
stimulants. Co nanoparticles (5 ppm) were the greatest in both proteinase production by the fungus as well as an
inducer of the proteolysis process by the enzyme when using faba bean straw as a proteinaceous substrate in the
reaction mixture, liberating the extreme quantity of amino acids, compared with the lack of the nanoparticles. The
findings suggest the incorporation of Co nanoparticles in both the proteinase fabrication process and during
the degradation of proteinaceous materials induce proteinase catalyst. This approach could be extended to modulate
the productivity and activity of similar biomolecules.},
DOI = {10.32604/biocell.2021.012011}
}