@Article{biocell.2021.013277,
AUTHOR = {ABDUR RAUF, SAUD BAWAZEER, MUSLIM RAZA, EMAN EL-SHARKAWY, MD. HABIBUR RAHMAN, MOHAMED A. EL-ESAWI, GHIAS UDDIN, BINA S. SIDDIQUI, ANEES AHMED KHALIL, JOSEPH MOLNAR, AKOS CSONKA, DIÁNA SZABÓ, HAROON KHAN, MOHAMMAD S. MUBARAK, TAIBI BEN HADDA, MUDYAWATI KAMARUDDIN, SEEMA PATEL},
TITLE = {Reversal of multidrug resistance and antitumor promoting activity of 3-oxo-6β-hydroxy- β-amyrin isolated from <i>Pistacia integerrima</i>},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {1},
PAGES = {139--147},
URL = {http://www.techscience.com/biocell/v45n1/41408},
ISSN = {1667-5746},
ABSTRACT = {The bioactive triterpenoid 3-oxo-6-β-hydroxy-β-amyrin (1) has been isolated from multiple plant sources. In this
study, chloroform fraction of Pistacia integerrima extract was processed for the isolation of the compound. The compound
identity was confirmed by advanced spectroscopy technique. X-ray crystallography was applied for molecular structure
confirmation. In addition, compound 1 was screen for its activity on reversal of MDR (multidrug resistance) mediated
by P-gp (P-glycoprotein). This was accomplished by using rhodamine123 exclusion on multidrug-resistant human
ABCB1 gene transfected mouse T-lymphoma cell line. Outcomes revealed that MDR reversing effect was comparable to
verapamil as positive control <i>in vitro</i>. Treatment of TPA-induced tumor promotion with 3-oxo-6β-hydroxy- β-amyrin
led to reduction in the applied anti-tumor promotion experiment. The chemo-preventive effect of 3-oxo-6β-hydroxy- β-
amyrin was comparable to curcumin as positive control based on the reduction of immediate early tumor antigen
expression. Molecular docking by applying Autodock Vina 1 and i-GEMDOCK v 2.1 tools indicated that compound 1
gives good docking results, as determined by their fitness score and specificity. Moreover, results showed that compound
1 isolated from <i>Pistacia integerrima</i> precisely attached to a region where co-crystallized ligand for receptor previously
existed. Our findings may explain the use of <i>Pistacia integerrima</i> plant extracts as an anticancer agent in folk medicine.},
DOI = {10.32604/biocell.2021.013277}
}