@Article{biocell.2021.013824,
AUTHOR = {POOJA BHADRECHA, MADHU BALA, VIKAS KAUSHIK, NASEEM A. GAUR, SIMRANJEET SINGH, JOGINDER SINGH, MANOJ KUMAR},
TITLE = {Folate-producing rhizobacteria of <i>Hippophae rhamnoides</i> L. from Indian trans-Himalaya low atmospheric zone},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {2},
PAGES = {387--394},
URL = {http://www.techscience.com/biocell/v45n2/41554},
ISSN = {1667-5746},
ABSTRACT = {<i>Hippophae rhamnoides</i> L. is a plant of immense ethnopharmacological importance and is a known source for
various valuable biochemicals and nutraceuticals. The production of folate, a vitamin involved in several vital functions, in
this plant is rather poorly understood. Herein, we investigate the hypothesis that rhizobial bacteria serve the plant in this
essential vitamin’s biosynthesis. Bacterial strains of <i>Bacillus</i>, <i>Azorhizobium</i>, <i>Frankia</i>, <i>Paenibacillus</i>, <i>Brevibacillus</i> and
<i>Pseudomonas</i>, were isolated from the rhizosphere of the plant. HPLC and LCMS were used to trace the production of
intra and extra-cellular folate by representative rhizospheric bacterial strains <i>in vitro</i>. From the seventeen functionally
characterized bacterial strains of the plant’s rhizosphere, thirteen produced significant amounts of folate.
<i>Azorhizobium</i> BR5401 produced the maximum amount of folic acid (424 µg/mL), and <i>Bacillus</i> GY779 was the only
strain capable of producing both intracellular and extra-cellular folic acid. The Open Reading Frame coding for
dihydroneopterin aldolase, an enzyme involved in folate biosynthesis, was found in one of the representative isolates.
Our experimental findings help us to suggest that the folate synthesized by rhizobial bacteria is transported to the
plant, highlighting a significant benefit of coexistence.},
DOI = {10.32604/biocell.2021.013824}
}