@Article{biocell.2021.014694,
AUTHOR = {TIECHENG SUN, YANDONG ZHANG, JIANHUA LI, HONG YU, LINGLI SONG, XINPING SUN, XI CHEN, LI TIAN},
TITLE = {Addition of peroxiredoxin 6 (PRDX6) to IVF fertilization medium maintains motility and longevity of human spermatozoa},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {3},
PAGES = {705--710},
URL = {http://www.techscience.com/biocell/v45n3/41681},
ISSN = {1667-5746},
ABSTRACT = {This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive
motility of human spermatozoa. Semen samples with normal parameters were collected from 23 males and
supplemented with different concentrations of peroxiredoxin 6. All the semen samples were measured according to
the WHO 5th manual, and the motile spermatozoa were extracted using IVF fertilization medium supplemented with
different peroxiredoxin 6 concentrations. Total motility and progressive motility were observed at different timepoints of culture at room temperature. After peroxiredoxin 6 supplementation, all groups had a significant increase in
total motility and progressive motility compared to the control group. The difference in total motility and progressive
motility between the 0 and 10−7 mM groups was observed at 24 and 48 h of culture at room temperature. At 24 h,
the total motility increased by 30% in the control group (16.03 ± 11.91 vs. 11.51 ± 7.84), and progressive motility
increased by 21% (10.53 ± 9.4 vs. 8.31 ± 6.04). A similar trend was observed in the 48 h group. In addition, we also
found that peroxiredoxin 6 had a well protective effect on sperm kinetic parameters at 10<sup>−7</sup> mM. The findings of this
study suggest that peroxiredoxin 6 can enhance sperm total motility and progressive motility in IVF fertilization
medium. Peroxiredoxin 6 may have potential benefits for sperm preparation in assisted reproductive technology.},
DOI = {10.32604/biocell.2021.014694}
}