@Article{biocell.2021.015039,
AUTHOR = {HANXIANZHI XIAO, RONGJIA QI, ZILING WANG, MINGHE XIAO, YUE XIANG, YAPING WANG, LU WANG},
TITLE = {<i>Angelica sinensis</i> polysaccharides ameliorate 5-flourouracil-induced bone marrow stromal cell proliferation inhibition via regulating Wnt/β-catenin signaling},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {4},
PAGES = {1045--1058},
URL = {http://www.techscience.com/biocell/v45n4/42361},
ISSN = {1667-5746},
ABSTRACT = {Chemotherapy may cause cellular oxidative stress to bone marrow. Oxidative damage of bone marrow
hematopoietic microenvironment is closely related to chronic myelosuppression after chemotherapeutic treatment.
<i>Angelica</i> sinensis polysaccharides (ASP) are major effective ingredients of traditional Chinese medicine <i>Angelica</i> with
multi-target anti-oxidative stress features. In the current study, we investigated the protective roles and mechanisms
of ASP on chemotherapy-induced bone marrow stromal cell (BMSC) damage. The human bone marrow stromal cell
line HS-5 cells were divided into control group, 5-FU group, 5-FU + ASP group, and 5-FU + LiCl group to
investigate the mechanism of ASP to alleviate 5-FU-induced BMSC proliferation inhibition. The results showed that
5-FU inhibits the growth of HS-5 cells in a time and dose-dependent manner; however, ASP partially counteracted
the 5-FU-induced decrease in cell viability, whereas Wnt signaling inhibitor Dkk1 antagonized the effect of ASP on
HS-5 cells. ASP reversed the decrease in total cytoplasmic β-catenin, p-GSK-3β, and CyclinD1 following 5-FU
treatment and modulated nuclear expression of β-catenin, Lef-1, and C-myc proteins. Furthermore, ASP also
enhanced the antioxidant capacity of cells and reduced 5-FU-induced oxidative stress, attenuated FoxO1 expression,
thus weakened its downstream apoptosis-related proteins and G0/G1 checkpoint-associated p27<sup>Kip1</sup> expression to
alleviate 5-FU-induced apoptosis and to promote cell cycle progression. All the results above suggest that the
protective role of ASP in 5-FU-treated BMSCs proliferation for the chemotherapy may be related to its activating
Wnt/β-catenin signaling and keeping homeostasis between β-catenin and FoxO1 under oxidative stress. The study
provides a potential therapeutic strategy for alleviating chemotherapeutic damage on BMSCs.},
DOI = {10.32604/biocell.2021.015039}
}