@Article{biocell.2021.015282,
AUTHOR = {QICHAO WANG, WUJING ZENG, BASHARAT ALI, XUEMIN ZHANG, LING XU, ZONGSUO LIANG},
TITLE = {Genome-wide identification of <i>WRKY</i> gene family and expression analysis under abiotic stresses in <i>Andrographis paniculata</i>},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {4},
PAGES = {1107--1119},
URL = {http://www.techscience.com/biocell/v45n4/42363},
ISSN = {1667-5746},
ABSTRACT = {<i>Andrographis paniculata</i> (<i>A. paniculata</i>) is a Chinese herbal medicine that clears away heat, reduces
inflammation, protects the liver, and promotes choleretics. The WRKYs of <i>A. paniculata</i> are still not well
characterized, although many WRKYs have been identified in various plant species. In the present study, 59 <i>A.
paniculata WRKY</i> (<i>ApWRKY</i>) genes were identified and renamed on the basis of their respective chromosome
distribution. These <i>ApWRKYs</i> were divided into three groups via phylogenetic analysis according to their WRKY
domains and combined with WRKY of Arabidopsis. The 59 identified <i>ApWRKY</i> transcription factors were nonuniformity distributed on 23 chromosomes of <i>A. paniculata</i>. From the structural analysis of the conserved motifs,
different <i>ApWRKYs</i> structures showed different biological functions, and the <i>ApWRKY</i> transcription factor had
certain species-specificity in the evolutionary process. The expression patterns of the 41 <i>ApWRKYs</i> were examined
through quantitative real-time PCR (qRT-PCR) in various tissues and under abiotic stresses (salt). The results showed
that most of the <i>ApWRKY</i> had different reactions to salt treatment. In addition, the content of the four main
secondary metabolites in <i>A. paniculata</i> leaves was determined under salt stress. The results show that under a low
concentration of salt treatment, the synthesis of andrographolide can be improved.},
DOI = {10.32604/biocell.2021.015282}
}