TY - EJOU
AU - WANG, QICHAO
AU - ZENG, WUJING
AU - ALI, BASHARAT
AU - ZHANG, XUEMIN
AU - XU, LING
AU - LIANG, ZONGSUO
TI - Genome-wide identification of WRKY gene family and expression analysis under abiotic stresses in Andrographis paniculata
T2 - BIOCELL
PY - 2021
VL - 45
IS - 4
SN - 1667-5746
AB - Andrographis paniculata (A. paniculata) is a Chinese herbal medicine that clears away heat, reduces
inflammation, protects the liver, and promotes choleretics. The WRKYs of A. paniculata are still not well
characterized, although many WRKYs have been identified in various plant species. In the present study, 59 A.
paniculata WRKY (ApWRKY) genes were identified and renamed on the basis of their respective chromosome
distribution. These ApWRKYs were divided into three groups via phylogenetic analysis according to their WRKY
domains and combined with WRKY of Arabidopsis. The 59 identified ApWRKY transcription factors were nonuniformity distributed on 23 chromosomes of A. paniculata. From the structural analysis of the conserved motifs,
different ApWRKYs structures showed different biological functions, and the ApWRKY transcription factor had
certain species-specificity in the evolutionary process. The expression patterns of the 41 ApWRKYs were examined
through quantitative real-time PCR (qRT-PCR) in various tissues and under abiotic stresses (salt). The results showed
that most of the ApWRKY had different reactions to salt treatment. In addition, the content of the four main
secondary metabolites in A. paniculata leaves was determined under salt stress. The results show that under a low
concentration of salt treatment, the synthesis of andrographolide can be improved.
KW - A. paniculata
KW - ApWRKY
KW - Secondary metabolites
KW - Diterpene lactones
KW - Andrographolide
KW - Abiotic stress
DO - 10.32604/biocell.2021.015282