@Article{biocell.2021.014336,
AUTHOR = {CHUNKUN FAN, YONGPENG ZHANG, CHUNBAO YANG, YAWEI TANG, JI QU, BU JIE, DEJI QUZHEN, LIYUN GAO},
TITLE = {Overexpression of rice F-box phloem protein gene <i>OsPP12-A13</i> confers salinity tolerance in <i>Arabidopsis</i>},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {4},
PAGES = {1121--1135},
URL = {http://www.techscience.com/biocell/v45n4/42365},
ISSN = {1667-5746},
ABSTRACT = {Salinity is a serious challenge for agriculture production by limiting the arable land. Rice is a major staple food
crop but very sensitive to salt stress. In this study, we used <i>Arabidopsis</i> for the functional characterization of a rice F-box
gene <i>LOC_Os04g48270</i> (<i>OsPP12-A13</i>) under salinity stress. <i>OsPP12-A13</i> is a nuclear-localized protein that is strongly upregulated under salinity stress in rice and showed the highest expression in the stem, followed by roots and leaves. Two
types of transgenic lines for <i>OsPP12-A13</i> were generated, including constitutive tissue over-expression using the
CaMV35S promoter and phloem specific over-expression using the pSUC2 promoter. Both types of transgenic plants
showed salinity tolerance at the seedling stage through higher germination percentage and longer root length, as
compared to control plants under salt stress in MS medium. Both the transgenic plants also exhibited salt tolerance at
the reproductive stage through higher survival rate, plant dry biomass, and seed yield per plant as compared to
control plants. Determination of Na<sup>+</sup> concentration in leaves, stem and roots of salt-stressed transgenic plants showed
that Na<sup>+</sup> concentration was less in leaf and stem as compared to roots. The opposite was observed in wild type
stressed plants, suggesting that <i>OsPP12-A13</i> may be involved in Na<sup>+</sup> transport from root to leaf. Transgenic plants
also displayed less ROS levels and higher activities of peroxidase and glutathione S-transferase along with upregulation
of their corresponding genes as compared to control plants which further indicated a role of <i>OsPP12-A13</i> in maintaining
ROS homeostasis under salt stress. Further, the non-significant difference between the transgenic lines obtained from the
two vectors highlighted that <i>OsPP12-A13</i> principally works in the phloem. Taken together, this study showed that
<i>OsPP12-A13</i> improves salt tolerance in rice, possibly by affecting Na<sup>+</sup> transport and ROS homeostasis.},
DOI = {10.32604/biocell.2021.014336}
}