TY - EJOU
AU - RUAN, YIBIN
AU - XIE, ZHONGMING
AU - LIU, QIONG
AU - ZHANG, LIXIAO
AU - HAN, XIKUI
AU - LIAO, XIAOYAN
AU - LIU, JIAN
AU - GAO, FENGGUANG
TI - Nicotine and menthol independently exert neuroprotective effects against cisplatin- or amyloid- toxicity by upregulating Bcl-xl via JNK activation in SH-SY5Y cells
T2 - BIOCELL
PY - 2021
VL - 45
IS - 4
SN - 1667-5746
AB - Nicotine and menthol, agonists of nicotinic acetylcholine receptor (nAChR) and transient receptor potential
melastatin type 8 (TRPM8), serve important roles in the prevention of cell death-involved neurodegenerative diseases.
However, the potential synergistic effects of nicotine and menthol on anti-apoptotic ability are still uncertain. In the
present study, the potential synergistic effects of nicotine and menthol on cisplatin or amyloid β1-42 induced cell
model of the neurodegenerative diseases were explored by assessing cell viability, TNF-α expression, caspase-3
activation, and the collapse of mitochondrial membrane potential in human SH-SY5Y neuroblastoma cells. Statistical
significance was tested using Student’s t-test or one-way ANOVA with post hoc Newman-Keuls test. The results
showed that: Firstly, SH-SY5Y cell viability was obviously increased by the treatments with nicotine and menthol.
Secondly, nicotine and menthol independently alleviated cisplatin or amyloid β1-42 induced TNF-α up-regulation.
Thirdly, nicotine and menthol abrogated the effect of cisplatin and amyloid β25-35 on caspase-3 activation.
Interestingly, the effect of cisplatin and amyloid β1-42 on the collapse of mitochondrial membrane potential was
efficiently attenuated by nicotine and menthol treatments. Most importantly, the inhibition of c-jun kinase (JNK)
activation abolished the effect of cisplatin, and amyloid β1-42 stimulated Bcl-xl expression. All these findings indicate
that nicotine and menthol independently exert neuroprotective effects by upregulating Bcl-xl via JNK activation.
Nicotine and menthol augmented Bcl-xl expression and JNK phosphorylation, and thus they are potential therapeutic
targets for altering the progress of neurodegenerative diseases.
KW - Nicotine
KW - Menthol
KW - Apoptosis
KW - Mitochondrial membrane potential
KW - Tumor necrosis factor-alpha
DO - 10.32604/biocell.2021.015261