@Article{biocell.2021.010119,
AUTHOR = {MENGYI TANG, BOWEN YANG, CHUANG ZHANG, CHAOXU ZHANG, DAN ZANG, LIBAO GONG, YUNPENG LIU, ZHI LI, XIUJUAN QU},
TITLE = {The <i>F5</i> gene predicts poor prognosis of patients with gastric cancer by promoting cell migration identified using a weighted gene co-expression network analysis},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {4},
PAGES = {911--921},
URL = {http://www.techscience.com/biocell/v45n4/42367},
ISSN = {1667-5746},
ABSTRACT = {Distal gastric cancer (DGC) is a subgroup of gastric cancer (GC), which has different molecular characteristics
from proximal gastric cancer (PGC). These differences result in different overall survival (OS) rates; however, data
pertaining to the survival rate in PGC or DGC are contradictory. This suggests that the location of GC is not the
unique cause of the different survival rates, while the molecular characteristics might be more important factors
determining the prognosis of DGC. Therefore, the aim of this study was to discover key prognostic factors in DGC
using bioinformatic methods and to explore the potential molecular mechanism. The Cancer Genome Atlas (TCGA)
public database was employed to screen data relating to DGC, and we conducted a weighted gene co-expression
network analysis (WGCNA) on DGC patient samples to establish co-expression modules. High-weight genes (hub
genes) in a dominant color module were identified. <i>In vitro</i> experiments and gene set enrichment analyses (GSEA)
were carried out to elucidate the potential molecular mechanism. In this study, 139 DGC samples were enrolled to
perform a co-expression analysis. According to the correlation between gene modules and clinical characteristics, the
royal blue module related to stage M of DGC was screened, and a survival analysis was conducted to show that highcoagulation-factor V (<i>F5</i>) expression was related to the short OS of patients with GC. <i>In vitro</i> experiments confirmed
that <i>F5</i> could promote the migration of GC cells. GSEA suggested that <i>F5</i> might have affected the prognosis of GC by
modulating the activities of the Wnt and/or the TGF-β signaling pathways. Our results indicated that high <i>F5</i>
expression predicts poor prognosis of patients with DGC, and it functions probably by promoting cell migration
through the Wnt and/or the TGF-β signaling pathways.},
DOI = {10.32604/biocell.2021.010119}
}