@Article{biocell.2021.013625,
AUTHOR = {XI YANG, KE MA, RUI CHEN, NAIYI ZHANG, YITING MENG, JIA WEN, QINPING LIAO},
TITLE = {Immunocytochemical examination of PTEN and Ki-67 for endometrial carcinoma using thin-layer endometrial preparations},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {4},
PAGES = {923--932},
URL = {http://www.techscience.com/biocell/v45n4/42368},
ISSN = {1667-5746},
ABSTRACT = {The current study is designed to evaluate certain immunocytochemical (ICC) biomarkers to gain a better
cytodiagnosis. For this purpose, 85 patients from March 2016 to March 2019 who planned to get a hysteroscopy assay
were recruited. Cytological sampling was conducted by scratching the uterus cavity using SAP-1, and the samples were
processed as liquid-based smears, using SurePath technology. 36 patients diagnosed with EC or atypical endometrial
hyperplasia were recruited in this study. 33 cases were diagnosed with EC, and 3 cases were diagnosed with atypical
endometrial hyperplasia, allocated with EC or precancerous lesions group. 26 cases were diagnosed with benign lesions
group. Among these cases, 9 cases were diagnosed with endometrial simple hyperplasia, 2 cases were diagnosed with
complicated hyperplasia, 5 cases were diagnosed with an irregular proliferation of endometrium and 10 cases were
diagnosed with endometrial polyps. There were 23 cases in the healthy group. Staining in thin-layer endometrial
preparations by ICC and using H-score or counting the percentage of stained cells. The presentation of PTEN in normal
endometrium, benign lesions, and EC/precancerous lesions were different (<i>p</i> < 0.01). Taking the cut-off value of 50
(Youden’s index: 0.698) PTEN expression for the diagnosis of EC/precancerous lesion, the sensitivity and specificity
were 83.7% and 86.1%. The presentation of Ki-67 in normal endometrium, benign lesions, and EC/precancerous lesions
were different (<i>p</i> < 0.01). Taking the cut-off value of 15% (Youden’s index: 0.76) Ki-67 expression for the diagnosis of
EC/precancerous lesion, the sensitivity and specificity were 94.4% and 81.6%. In this study, the use of different cut-off
values for Ki-67 and PTEN helped differentiate endometrial lesions. Immunocytochemistry in the ECT detection of
PTEN and Ki-67 can improve the diagnostic capabilities of endometrial cancer and precancerous lesions.},
DOI = {10.32604/biocell.2021.013625}
}