@Article{biocell.2021.015771,
AUTHOR = {HAZAEL RAMIRO CEJA-GALVEZ, ERANDIS DHENI TORRES-SÁNCHEZ, JUAN HERIBERTO TORRES-JASSO, EMMANUEL REYESURIBE, JOEL SALAZAR-FLORES},
TITLE = {Relationship between PON-1 enzymatic activity and risk factors for pesticide poisoning in farmers from the Cienega, Jalisco, Mexico},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {5},
PAGES = {1241--1250},
URL = {http://www.techscience.com/biocell/v45n5/43078},
ISSN = {1667-5746},
ABSTRACT = {Paraoxonase-1 (PON-1) is an enzyme that hydrolyzes organophosphate pesticides. The presence of
polymorphisms in PON-1 (L55M and Q192R) decreases its enzyme activity and increases the risk of central nervous
system (CNS) toxicity in occupationally exposed farmers, leading to chronic degenerative diseases and death. We
studied 103 farmers in the region of Cienega Jalisco, Mexico, which were exposed mainly to organophosphate
pesticides. We used serum and plasma samples to assay PON-1 activity and perform polymorphism analysis (L55M
and Q192R) using qPCR and TaqMan probes, respectively. For both polymorphisms, there was high percentage of
heterozygosity (55 LL = 0.19, LM = 0.75, MM = 0.06; 192 QQ = 0.12, QR = 0.72, RR = 0.16), while the allelic
frequencies were more balanced (L = 0.56, M = 0.44; Q = 0.48, R = 0.52). There were no significant differences in
enzyme activity of L55M polymorphism genotypes (LL = 179.27; LM = 192.11; MM = 122.11; QQ = 135.74; QR =
187.90; RR = 209; <i>p</i> > 0.05). But there was a slight decrease in enzyme activity for the Q192R polymorphism
genotypes. The genotype and alcohol consumption associated with slight increases in enzyme activity. However,
genotype and tobacco consumption did not have a significant effect on PON-1 activity (µU/mL) (<i>p</i> > 0.05). Overall,
alcohol and tobacco consumption affected PON-1 enzyme activity (µU/mL) up to 21.1%. The data obtained in this
study reveal that PON-1 activity is affected by genetic variants such as Q192R and alcohol consumption. This may
influence the susceptibility of populations to organophosphate poisoning.},
DOI = {10.32604/biocell.2021.015771}
}