TY  - EJOU
AU  - JEBERSON, MUNIYANDI SAMUEL 
AU  - CHAUDHARY, HARINDER KUMAR 
AU  - CHAHOTA, RAKESH KUMAR 
AU  - WANI, SHABIR HUSSAIN 

TI  - Doubled haploid production in advanced back cross generations and molecular cytogenetic characterization of rye chromatin in triticale × wheat derived doubled haploid lines
T2  - BIOCELL

PY  - 2021
VL  - 45
IS  - 6
SN  - 1667-5746

AB  - The rye genome has shown potential for improvement of bread wheat, where wheat-rye substitutions and translocations have been and are frequently used in resistance breeding. Crosses belongs to different generations viz., BC<sub>1</sub>F<sub>1</sub>, BC<sub>1</sub>F<sub>2</sub>, BC<sub>1</sub>F<sub>3</sub>, BC<sub>1</sub>F<sub>4</sub> and BC<sub>2</sub>F<sub>3</sub> of triticale × wheat derived were used for different haploid induction parameters using Gogon grass (<i>Imperata cylindrica)</i> as a pollen source. The percentage of pseudo seed formation ranged from 34.55% for BC<sub>1</sub>F<sub>2</sub> to 63.77 for BC<sub>1</sub>F<sub>1</sub> crosses, the haploid embryo formation ranges from 9.43% for BC<sub>1</sub>F<sub>1</sub> to 30.2% for BC<sub>1</sub>F<sub>2</sub>, the haploid plant generation ranges from 19.36% for BC<sub>1</sub>F<sub>2</sub> to 63.25% for BC<sub>1</sub>F<sub>1</sub>. Four doubled haploids were developed from ITSN 105/58 × VL 802 × VL 802 of BC<sub>2</sub>F<sub>3</sub> underwent molecular cytogenetic analyses using the probes, viz., rye genomic rDNA, pSc 119 and pAs1. FISH and GISH analysis revealed an IBL.1RS translocation and substitution of 5R chromosome instead of the 5D chromosomes in these doubled haploids.
KW  - Haploid; Triticale <i>×</i> wheat backcross; FISH; GISH; Rye chromatin

DO  - 10.32604/biocell.2021.015735