@Article{biocell.2021.016121,
AUTHOR = {SAMUELE GRECO, ELISA D’AGOSTINO, CHIARA MANFRIN, ANASTASIA SERENA GAETANO, GAEL FURLANIS, FRANCESCA CAPANNI, GIANFRANCO SANTOVITO, PAOLO EDOMI, PIERO GIULIO GIULIANINI, MARCO GERDOL},
TITLE = {RNA-sequencing indicates high hemocyanin expression as a key strategy for cold adaptation in the Antarctic amphipod Eusirus cf. giganteus clade g3},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {6},
PAGES = {1611--1619},
URL = {http://www.techscience.com/biocell/v45n6/44272},
ISSN = {1667-5746},
ABSTRACT = {We here report the de novo transcriptome assembly and functional annotation of Eusirus cf. giganteus clade g3,
providing the first database of expressed sequences from this giant Antarctic amphipod. RNA-sequencing, carried out on
the whole body of a single juvenile individual likely undergoing molting, revealed the dominant expression of
hemocyanins. The mRNAs encoding these oxygen-binding proteins cumulatively accounted for about 40% of the total
transcriptional effort, highlighting the key biological importance of high hemocyanin production in this Antarctic
amphipod species. We speculate that this observation may mirror a strategy previously described in Antarctic
cephalopods, which compensates for the decreased ability to release oxygen to peripheral tissues at sub-zero
temperatures by massively increasing total blood hemocyanin content compared with temperate species. These
preliminary results will undoubtedly require confirmation through proteomic and biochemical analyses aimed at
characterizing the oxygen-binding properties of E. cf. giganteus clade g3 hemocyanins and at investigating whether
other Antarctic arthropod species exploit similar adaptations to cope with the challenges posed by the extreme
conditions of the polar environment.},
DOI = {10.32604/biocell.2021.016121}
}