@Article{biocell.2021.016316,
AUTHOR = {CÜNEYT UÇARLI},
TITLE = {Genome-wide identification and<i> in silico</i> gene expression analysis of the related to ABI3/VP1 (RAV) transcription factor family in barley (<i>Hordeum vulgare</i> L.)},
JOURNAL = {BIOCELL},
VOLUME = {45},
YEAR = {2021},
NUMBER = {6},
PAGES = {1673--1685},
URL = {http://www.techscience.com/biocell/v45n6/44277},
ISSN = {1667-5746},
ABSTRACT = {RAV (Related to ABI3/VP1) transcription factors are unique members of the AP2-ERF superfamily with AP2
and B3 domains and play important roles in the regulation of seed germination, plant growth, and stress response. In the
study, 7 RAV genes, named <i>HvRAVs</i>, were identified in barley based on the available genome sequences. While five of the
seven <i>HvRAVs</i> were located on chromosome 3, <i>HvRAV5</i> and <i>HvRAV7 </i>were located on chromosome 1 and 4, respectively.
Six of the predicted <i>HvRAVs</i> were intron-less, except <i>HvRAV2</i>, which had one intron. HvRAV proteins have shown basic,
instable, and hydrophilic properties. The AP2 domain specific RAYD and WLG motifs were detected in all HvRAV
proteins. Besides, B3 repression domain, R/KLFGV, is also found in the C-terminal of HvRAVs. <i>HvRAVs</i> were found
to have stress-related cis-acting elements, including MYB, MYC, and W-BOX. <i>HvRAV2</i> was predicted to have no
GARE motifs, TATCCCA or TAACAA(G/A), and LTREs. Under drought conditions, the expression level of <i>HvRAVs</i>
did not significantly change in a drought-sensitive barley genotype, whereas <i>HvRAV5</i> and <i>HvRAV7</i> were dramatically
down-regulated in a drought-tolerant genotype. Expression of <i>HvRAV5</i> was also inhibited by salinity. <i>HvRAV7</i> was
strongly induced by plant pathogen attack. Only <i>HvRAV6</i> was induced by exogenous gibberellin application and
during the germination process. Interestingly, <i>HvRAV6</i> transcript was detected higher than other <i>HvRAVs</i> in all stress
and control conditions as well as during germination. <i>In silico</i> analyses have shown that <i>HvRAVs</i> play a role in
response to different abiotic and biotic stresses as well as in plant development. However, the extensive biological
roles of <i>HvRAV</i> genes in plant development and in response to abiotic and biotic stresses need further investigation.},
DOI = {10.32604/biocell.2021.016316}
}