@Article{biocell.2022.016563,
AUTHOR = {JUAN ZHANG, JUN HUANG, YAYUAN TANG, XIUZHEN LONG},
TITLE = {Transcriptome profile analysis of the accompanying migratory parasitic wasp <i>Aenasius bambawalei</i> (= <i>Aenasius arizonensis</i> girault) (Hymenoptera: Encyrtidae): Genes related to fertilization involved at different stage of ovary development},
JOURNAL = {BIOCELL},
VOLUME = {46},
YEAR = {2022},
NUMBER = {1},
PAGES = {195--205},
URL = {http://www.techscience.com/biocell/v46n1/44753},
ISSN = {1667-5746},
ABSTRACT = {Age-related declines in fertilization success have been reported for a wide range of species. The fertilization of parasitic wasps is closely related to egg production and sperm storage. <i>Aenasius bambawalei</i> (Hymenoptera: Encyridae) is a key parasitic wasp of the important invasive mealybug <i>Phenacoccus solenopsis</i> (Hemiptera: Pseudococcidae). The female offspring ratio of this parasitic wasp was declined with parental age in mass rearing under laboratory conditions. To investigate the molecular mechanisms involved in the reproduction of <i>A. bambawalei</i>, an extensive analysis of the impact of age on transcriptome profile of mated ovaries of this wasp was performed by comparing the gene expression profiles of various maternal ages: the early stage (ES), the intermediate stage (IS), and the advanced stage (AS). In total, 358 differentially expressed genes were identified, with 17.60% (63 genes) of the changes associated with greater expression in fertilization. Moreover, the expression of <i>serine protease</i> 47 <i>precursor</i>, <i>serine protease inhibitor</i> 3/4, <i>glucose dehydrogenase</i>, <i>fatty acyl-CoA reductase</i> 1-<i>like</i>, <i>major royal jelly</i>, and <i>acyl-CoA delta</i> (11) <i>desaturase-like</i> was detected by real-time fluorescence quantitative (RT-qPCR). The results showed that fertilization related genes exhibited a stage-specific pattern. Egg production and sperm storage genes in <i>A. bambawalei</i> were significantly modified in the transcriptome, providing a starting point for the genetic dissection of fertilization.},
DOI = {10.32604/biocell.2022.016563}
}