@Article{biocell.2021.015932,
AUTHOR = {AASTHA MITTAL, NEELAM MAHALA, KOWTHAVARAPU VENKATA KRISHNA, UMA S. DUBEY, SUNIL KUMAR DUBEY},
TITLE = {Calcium chloride linked camel milk derived casein nanoparticles for the delivery of sorafenib in hepatocarcinoma cells},
JOURNAL = {BIOCELL},
VOLUME = {46},
YEAR = {2022},
NUMBER = {1},
PAGES = {127--136},
URL = {http://www.techscience.com/biocell/v46n1/44763},
ISSN = {1667-5746},
ABSTRACT = {Sorafenib, a multikinase inhibitor used for the treatment of hepatocellular carcinoma, is limited by its low oral
bioavailability. To overcome this drawback, we have developed novel camel milk casein-derived nanoparticles as a drug
delivery system. Camel milk casein is not only biocompatible on oral administration but is actually a dietary protein of
pharmaceutical relevance. Casein is used because of its amphiphilic nature, self-assembling property, ability to show
sustained release, and capability of encapsulating both hydrophilic and hydrophobic drugs. In this study, camel milk
casein nanoparticles loaded with sorafenib were developed and characterized. Characterization of casein nanoparticles
was done by dynamic light scattering (DLS), zeta potential analysis, scanning light microscopy (SEM), and FTIR. The
drug content in nanoparticle and drug-protein binding studies were conducted by UV spectroscopy. The cytotoxicity
and cellular uptake efficiency studies were performed in HepG2 cell lines. It was observed that the cytotoxic effect of
sorafenib loaded camel milk casein nanoparticles was more than free sorafenib in HepG2 cells. This work suggests
camel milk casein as a suitable drug delivery molecule for sorafenib. In the future, it may also be used in enhancing
the efficacy and specific distribution of other water-insoluble anticancer drugs.},
DOI = {10.32604/biocell.2021.015932}
}