@Article{biocell.2022.021542,
AUTHOR = {LEI WANG, QIUXIA DU, YISU SHI, MICHAEL ACKAH, PENG GUO, DANYAN ZHENG, MENGMENG WU, XIN JIN, PEILAN LI, QIAONAN ZHANG, RUIXUE LI, ZHI YIN, MENGDI ZHAO, WEIGUO ZHAO},
TITLE = {Response of <i>MaHMA2</i> gene expression and stress tolerance to zinc stress in mulberry (<i>Morus alba</i> L.)},
JOURNAL = {BIOCELL},
VOLUME = {46},
YEAR = {2022},
NUMBER = {10},
PAGES = {2327--2342},
URL = {http://www.techscience.com/biocell/v46n10/48214},
ISSN = {1667-5746},
ABSTRACT = {<i>HMA2</i> (heavy metal ATPase 2) plays a crucial role in extracellular and intracellular Zn<sup>2+</sup> transport across biomembranes, maintaining ion homeostasis, and playing an important role in the normal physiological metabolism, growth, and development of plants. In our study, a novel <i>HMA2</i> gene, named <i>MaHMA2</i>, was isolated and cloned from white mulberry (<i>Morus alba</i> L.). The gene sequence obtained was 1,342 bp long, with an open reading frame of 1,194 bp, encoding a protein of 397 amino acids, with a predicted molecular mass of 42.852 kD and an isoelectric point of 7.53. This protein belonged to the PIB-type ATPase transport protein family. We analyzed the expression of the <i>MaHMA2</i> gene by quantitative real-time PCR. The results showed that the level of <i>MaHMA2</i> gene expression decreased to a Zn concentration of 800 mg/kg. Malondialdehyde and proline levels increased and responded to increasing Zn when the <i>MaHMA2</i> gene was silenced, whereas the activities of peroxidase and superoxide dismutase tended to increase in response to increasing Zn<sup>2+</sup> ion stress concentrations but were lower in the gene-silenced plants. These findings suggested that the <i>MaHMA2</i> gene played an active role in the tolerance response of mulberry to Zn stress.},
DOI = {10.32604/biocell.2022.021542}
}