@Article{biocell.2021.015701,
AUTHOR = {XINWEI WU, GUOYONG JIA, HONGNA YANG, CONGCONG SUN, YING LIU, ZENGYAN DIAO},
TITLE = {Neural stem cell-conditioned medium upregulated the PCMT1 expression and inhibited the phosphorylation of MST1 in SH-SY5Y cells induced by Aβ<sub>25-35</sub>},
JOURNAL = {BIOCELL},
VOLUME = {46},
YEAR = {2022},
NUMBER = {2},
PAGES = {471--478},
URL = {http://www.techscience.com/biocell/v46n2/45103},
ISSN = {1667-5746},
ABSTRACT = {A progressive neurodegenerative disease, Alzheimer’s disease (AD). Studies suggest that highly expressed
protein isoaspartate methyltransferase 1 (PCMT1) in brain tissue. In the current study, we explored the effects of
neural stem cell-conditioned medium (NSC-CDM) on the PCMT1/MST1 pathway to alleviate Aβ<sub>25-35</sub>-induced
damage in SH-SY5Y cells. Our data suggested that Aβ25-35 markedly inhibited cell viability. NSC-CDM or Neural
stem cell-complete medium (NSC-CPM) had a suppression effect on toxicity when treatment with Aβ<sub>25-35</sub>, with a
greater effect observed with NSC-CDM. Aβ<sub>25-35</sub> + NSC-CDM group exhibited an increase in PCMT1 expression.
sh-PCMT1 markedly decreased cell proliferation and suppressed the protective role of NSC-CDM through the
induction of apoptosis and improved p-MST1 expression. Overexpression of PCMT1 reversed the Aβ<sub>25-35</sub>-induced
decrease in cell proliferation and apoptosis. In summary, our findings suggest that NSC-CDM corrects the Aβ<sub>25-35</sub>-
induced damage to cells by improving PCMT1 expressions, which in turn reduces phosphorylation of MST1.},
DOI = {10.32604/biocell.2021.015701}
}