TY  - EJOU
AU  - WU, XINWEI 
AU  - JIA, GUOYONG 
AU  - YANG, HONGNA 
AU  - SUN, CONGCONG 
AU  - LIU, YING 
AU  - DIAO, ZENGYAN 

TI  - Neural stem cell-conditioned medium upregulated the PCMT1 expression and inhibited the phosphorylation of MST1 in SH-SY5Y cells induced by Aβ<sub>25-35</sub>
T2  - BIOCELL

PY  - 2022
VL  - 46
IS  - 2
SN  - 1667-5746

AB  - A progressive neurodegenerative disease, Alzheimer’s disease (AD). Studies suggest that highly expressed
protein isoaspartate methyltransferase 1 (PCMT1) in brain tissue. In the current study, we explored the effects of
neural stem cell-conditioned medium (NSC-CDM) on the PCMT1/MST1 pathway to alleviate Aβ<sub>25-35</sub>-induced
damage in SH-SY5Y cells. Our data suggested that Aβ25-35 markedly inhibited cell viability. NSC-CDM or Neural
stem cell-complete medium (NSC-CPM) had a suppression effect on toxicity when treatment with Aβ<sub>25-35</sub>, with a
greater effect observed with NSC-CDM. Aβ<sub>25-35</sub> + NSC-CDM group exhibited an increase in PCMT1 expression.
sh-PCMT1 markedly decreased cell proliferation and suppressed the protective role of NSC-CDM through the
induction of apoptosis and improved p-MST1 expression. Overexpression of PCMT1 reversed the Aβ<sub>25-35</sub>-induced
decrease in cell proliferation and apoptosis. In summary, our findings suggest that NSC-CDM corrects the Aβ<sub>25-35</sub>-
induced damage to cells by improving PCMT1 expressions, which in turn reduces phosphorylation of MST1.
KW  - Neural stem cell conditioned medium
KW  -  Protein isoaspartate methyltransferase 1
KW  -  MST1
KW  -  Amyloid β<sub>25-35</sub>
KW  -  Apoptosis

DO  - 10.32604/biocell.2021.015701