@Article{biocell.2022.017437,
AUTHOR = {HUIZE CHEN, XINGTIAN JIA, HAIYAN RAN, JINGRONG NIU, MEITING DU},
TITLE = {Application of VIGS system to explore the function of common wheat TaADF7},
JOURNAL = {BIOCELL},
VOLUME = {46},
YEAR = {2022},
NUMBER = {2},
PAGES = {559--565},
URL = {http://www.techscience.com/biocell/v46n2/45116},
ISSN = {1667-5746},
ABSTRACT = {As an allohexaploid plant, common wheat has a complex gene structure, making it difficult to study its gene function. Virus-induced gene silencing (VIGS) is an important tool for the rapid analysis of plant gene function. In this study, the gene silencing system, namely, barley stripe mosaic virus (BSMV)-VIGS induced by BSMV was used to silence the wheat phytoene desaturase (<i>TaPDS</i>) and actin depolymerization factor (<i>TaADF7</i>) genes and determine the effect of gene silencing on wheat. <i>TaPDS</i> was used as an indicator gene to determine the feasibility of VIGS system, while <i>TaADF7</i> was used as a test gene to determine its effect on wheat growth. Results showed that the leaves of tobacco and wheat were bleached by the mixture of <i>pCaBS-α</i>, <i>pCaBS-β</i>, and <i>pCaBS-γ::TaPDS</i>, indicating that the <i>TaPDS</i> gene was silenced, and the bleached leaves had physiological activity as determined by trypan blue staining. Therefore, the VIGS system was efficient and available. After the tobacco was treated with <i>pCaBS-α</i>, <i>pCaBS-β</i>, and <i>pCaBS-γ::TaADF7</i>, the viral suspension was obtained. The expression of <i>TaADF7</i> gene was downregulated after wheat leaves were infected by friction, indicating that the expression of <i>TaADF7</i> was silenced. Laser confocal scanning microscopy showed that the silencing of <i>TaADF7</i> enhanced the fluorescence of microfilament skeleton in mesophyll protoplasts and significantly reduced the plant height. Results showed that <i>TaADF7</i> affected cell division and plant growth by inhibiting microfilament depolymerization. In conclusion, the BSMV-VIGS system was used to silence wheat <i>TaPDS</i> and <i>TaADF7</i> genes. Bleaching phenomenon was observed in wheat leaves after <i>TaPDS</i> silencing. After <i>TaADF7</i> silencing, microfilaments in wheat mesophyll cells gathered into coarse bundles, which affected the dynamics of microfilaments and inhibited plant growth.},
DOI = {10.32604/biocell.2022.017437}
}