TY  - EJOU
AU  - MATSUSHITA, YUKI 
AU  - ONO, WANIDA 
AU  - ONO, NORIAKI 

TI  - Synergy of single-cell sequencing analyses and <i>in vivo</i> lineage-tracing approaches: A new opportunity for stem cell biology
T2  - BIOCELL

PY  - 2022
VL  - 46
IS  - 5
SN  - 1667-5746

AB  - Single-cell sequencing technologies have rapidly progressed in recent years, and been applied to characterize stem cells in a number of organs. Somatic (postnatal) stem cells are generally identified using combinations of cell surface markers and transcription factors. However, it has been challenging to define micro-heterogeneity within “stem cell” populations, each of which stands at a different level of differentiation. As stem cells become defined at a single-cell level, their differentiation path becomes clearly defined. Here, this viewpoint discusses the potential synergy of single-cell sequencing analyses with <i>in vivo</i> lineage-tracing approaches, with an emphasis on practical considerations in stem cell biology.
KW  - Single-cell sequencing; <i>in vivo</i> lineage-lineage; skeletal stem cells (SSCs); mesenchymal stem cells (MSCs); bone marrow stromal cells (BMSCs)

DO  - 10.32604/biocell.2022.018960