TY  - EJOU
AU  - LI, HE 
AU  - CHEN, RUNAN 
AU  - CHEN, ZHONGG 
AU  - LIN, JIAXIN 
AU  - JIN, XIJUN 
AU  - REN, CHUNYUAN 
AU  - CHEN, QIUSEN 
AU  - CHEN, FENGQIONG 
AU  - YU, GAOBO 
AU  - ZHANG, YUXIAN 

TI  - The molecular characteristics of soybean ARR-B transcription factors
T2  - BIOCELL

PY  - 2022
VL  - 46
IS  - 6
SN  - 1667-5746

AB  - The Type-B authentic response regulator (ARR-Bs) gene family is one of the important plant-specific transcription factor families involved in variety of physiological processes. However, study of ARR-Bs gene family in soybean is limited. Genome-wide analysis and expression profiling of the ARR-Bs gene family were performed in the soybean genome. 31 ARR-Bs genes (namely GmARR-B1-31) were identified, containing conserved catalytic domains with protein lengths and molecular weights ranging from 246 to 699 amino acids (aa) and 28.30 to 76.86 kDa, respectively. Phylogenetic analysis grouped ARR-Bs genes into three clusters—Cluster I, Cluster II, and Cluster III—which included 15, 12, and 4 genes, respectively, and were asymmetrically distributed on 17 chromosomes. Tissue specific expression analysis of GmARR-Bs family revealed a high transcription level in flowers, roots and seeds. The subcellular localization of GmARR5, GmARR14 were observed in the nucleus, and the promoter region of them included low-temperature responsive element (LTR), the C-repeat/dehydration responsive element (DRE), MBS, ABRE, MYB, MEJA and TCA-elements, which possibly participate in abiotic stress and hormones responses. qRT-PCR analysis showed that the expression of GmARR-B genes were affected by different abiotic stresses, especially cold stress and salt stress, and GmARR-B5 and GmARR-B14 were significantly induced by cold stress. This suggested that ARR-Bs genes were involved in multiple abiotic stress response pathways and acted as a positive factor under cold stress.
KW  - Soybean; Type-B authentic response regulator (B-ARRs); Abiotic stress

DO  - 10.32604/biocell.2022.018762