@Article{biocell.2022.016564,
AUTHOR = {MIN CHEN, YUAN HUANG, WEN XU, CHUNLIN SU},
TITLE = {Intrauterine high androgen promotes obesity of the offspring of rats with polycystic ovarian syndrome via activating macrophage-angiogenesis-related androgen signaling},
JOURNAL = {BIOCELL},
VOLUME = {46},
YEAR = {2022},
NUMBER = {6},
PAGES = {1505--1519},
URL = {http://www.techscience.com/biocell/v46n6/46703},
ISSN = {1667-5746},
ABSTRACT = {The development of polycystic ovary syndrome (PCOS) is closely related to the chronic inflammatory and
obese. Recent studies have found macrophages regulate the chronic inflammation and adipose tissue remodelling, but
the underlying mechanisms have not been clarified. In this study, we established a model of PCOS in the offspring
rats by high androgen exposure during late pregnancy in parental and established a female rat macrophage
eliminating model by rejection of clodronate liposome. Then, the offspring rat macrophage phenotype in offspring
female rat adipose tissue, and levels of testosterone, angiogenic factors (PDGF and VEGF) and inflammatory factors
(TNF-α and MCP-1) were investigated. By coculture of RAW264.7 macrophage with adipocytes or C166 endothelial
cells (ECs), the mobility of adipocytes, and the ECs function with associated signalling pathway were detected by
using of androgen inhibitor Apalutamide, NF-κB inhibitor JSH-23 and ERK1/2 inhibitor LY3214996. It was found
that high androgen exposure during late pregnancy led to increased testosterone levels and overweight and obesity,
increased size and reduced number of subcutaneous and intra-abdominal adipocytes, and increased secretion of
TNF-α and MCP-1 in female rats in the offspring. Eliminating macrophages significantly increased adipocytes and
angiogenesis in offspring of rats with intrauterine high androgen, and reduced TNF-α and MCP-1. Macrophages
promoted mobility of adipocytes, and inhibited proliferation, migration, tube formation of ECs under
hyperandrogenic condition, which were significantly inhibited by Apalutamide, JSH-23 and LY3214996. Thus,
intrauterine high androgen promotes obesity of the offspring of rats with polycystic ovarian syndrome through
increasing M1 differentiation of pro-inflammatory macrophages and activating VEGF-related angiogenesis via
androgen/NF-κB/ERK1/2 signalling pathway.},
DOI = {10.32604/biocell.2022.016564}
}