@Article{biocell.2022.019111,
AUTHOR = {SITI NABILAH; TRI HANDOYO; KYUNG-MIN KIM; MOHAMMAD UBAIDILLAH},
TITLE = {Expression analysis of <i>OsSERK</i>, <i>OsLEC1</i> and <i>OsWOX4</i> genes in rice (<i>Oryza</i> sativa L.) callus during somatic embryo development},
JOURNAL = {BIOCELL},
VOLUME = {46},
YEAR = {2022},
NUMBER = {7},
PAGES = {1633--1641},
URL = {http://www.techscience.com/biocell/v46n7/47028},
ISSN = {1667-5746},
ABSTRACT = {Somatic embryogenesis is an asexual reproduction process that occurs in many plant species, including rice. 
This process contains several totipotency markers such as Somatic Embryogenesis Receptor-like Kinase (SERK), Leafy 
Cotyledon1 (LEC1) and WUSCHEL-Related Homeobox4 (WOX4) and also a helpful model for embryo development 
and clones and transformations. Here, we report the gene expression during somatic embryo development correlates 
with regeneration frequency in 14 Javanica rice (pigmented and non-pigmented) using modifified N6 media 
supplemented with Kinetin (2.0 mg/L) and NAA (1.0 mg/L). Although there have been advances in understanding 
the genetic basis of somatic embryogenesis in other varieties, rice is still unexplored, especially during somatic embryo 
development. Moreover, for the formation of callus induction from immature embryos, 2,4-D (2.0 mg/L, 3.0 mg/L) 
was used. This study analysed the gene expression of <i>OsSERK</i>, <i>OsWOX4</i> and <i>OsLEC1</i> genes through RT-PCR analysis. 
Higher expression of the <i>OsLEC1</i> gene indicates that their function may correlate in the in vitro with the high 
response of rice after transfer to regeneration media. This study found that rice varieties of pigmented rice (MS 
Pendek and Gogoniti II) and non-pigmented rice (Pandan Ungu) showed high regeneration frequency, showing 
higher <i>OsLEC1</i> expression than other varieties because <i>OsLEC1</i> promotes the maturation of somatic embryos in plant 
regeneration on day 14. However, the contrast with Genjah nganjuk may be effective because of other regulatory 
genes. RT-PCR analysis showed <i>OsSERK</i> had less expression level than <i>OsLEC1</i> and <i>OsWOX4</i> in the varieties, which 
correlate with the percentage of plant regeneration, but not for Gogoniti II. In conclusion, the higher percentage of 
plant regeneration correlates with the higher expression level of <i>OsLEC1</i> at day 14 of media regeneration of rice.
},
DOI = {10.32604/biocell.2022.019111}
}