@Article{biocell.2022.020391,
AUTHOR = {MING-AO CAO, FEI ZHANG, ELSAYED FATHI ABD_ALLAH, QIANGSHENG WU},
TITLE = {Mycorrhiza improves cold tolerance of Satsuma orange by inducing antioxidant enzyme gene expression},
JOURNAL = {BIOCELL},
VOLUME = {46},
YEAR = {2022},
NUMBER = {8},
PAGES = {1959--1966},
URL = {http://www.techscience.com/biocell/v46n8/47587},
ISSN = {1667-5746},
ABSTRACT = {A potted experiment was carried out to study the effect of an arbuscular mycorrhizal fungus (<i>Diversispora
versiformis</i>) and arbuscular mycorrhizal like fungus (<i>Piriformospora indica</i>) on antioxidant enzyme defense system of
Satsuma orange (<i>Citrus sinensis</i> cv. Oita 4) grafted on Poncirus trifoliata under favourable temperature (25°C) and
cold temperature (0°C) for 12 h. Such short-term treatment of cold temperature did not cause any significant change
in root fungal colonization and spore density in soil. Under cold stress, <i>D. versiformis </i>inoculation did not change the
activity of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in leaves and roots, whereas P. indica
inoculation significantly increased the activity of CAT in roots and POD in leaves only. In addition, inoculation of
two mycorrhizal fungi under cold stress significantly increased the relative expression levels of <i>PtPOD</i> and <i>PtF-SOD</i>
in leaves, <i>P. indica</i> up-regulated the expression levels of<i> PtCu/Zn-SOD</i> in leaves, and <i>D. versiformis</i> also induced the
expression levels of <i>PtMn-SOD</i> and <i>PtCAT1</i> in leaves. In addition, inoculated Oita 4 trees maintained significantly
lower hydrogen peroxide levels and malondialdehyde contents in leaves and roots under cold temperature, suggesting
lower oxidative damage. Therefore, we concluded that arbuscular mycorrhizal fungi (especially <i>P. indica</i>) mainly
induced the expression of antioxidant enzyme genes, depending on the fungal species, and thus mitigated oxidative
damage for higher cold resistance in inoculated plants.},
DOI = {10.32604/biocell.2022.020391}
}