@Article{biocell.2022.019807,
AUTHOR = {EUN NA, SUN YOUNG LIM},
TITLE = {Effect of <i>Lycopus lucidus</i> Turcz. supplementation on gut microflora and short chain fatty acid composition in Crj: CD-1 mice},
JOURNAL = {BIOCELL},
VOLUME = {46},
YEAR = {2022},
NUMBER = {9},
PAGES = {2101--2109},
URL = {http://www.techscience.com/biocell/v46n9/47743},
ISSN = {1667-5746},
ABSTRACT = {We investigated the diversity and composition of microflora in feces of Lycopus lucidus Turcz.-fed mice. In
addition, we evaluated the production of major cytokines (Interleukin-6 and -10) which are related to inflammation
and fatty acid composition of several tissues. 16S ribosomal DNA sequencing-based microbiome taxonomic profiling
analysis was performed utilizing the EzBioCloud data base. Male mice fed on <i>L. lucidus</i> showed a significantly
reduced number of lactic acid bacteria and coliform in the feces compared with the control group (<i>p</i> < 0.05). 16S
rDNA sequencing analysis of fecal samples showed that <i>L. lucidus</i> supplementation decreased the community of
harmful microflora (<i>Enterobacteriaceae</i> including <i>Escherichia coli</i> and <i>Bacteroides</i> sp.) in feces compared with the
control group (p < 0.05). There were no significant differences in mRNA expression of cytokine IL-6 and IL-10
between the control and <i>L. lucidus</i> fed groups. The fecal fatty acid composition in the <i>L. lucidus</i> group had
percentages of 4:0, 6:0, 8:0 and 10:0 in the intestine but those short chain fatty acids were not detected in the control
group. Our results showed that <i>L. lucidus</i> supplementation influenced gut environment by decreasing harmful
microflora and increased the percentages of several short fatty acids.},
DOI = {10.32604/biocell.2022.019807}
}