@Article{biocell.2022.020069,
AUTHOR = {XIAOFANG WANG, XIAOLIN TU, YUFEI MA, JIE CHEN, YANG SONG, GUANGLIANG LIU},
TITLE = {Wnt3a-induced ST2 decellularized matrix ornamented PCL scaffold for bone tissue engineering},
JOURNAL = {BIOCELL},
VOLUME = {46},
YEAR = {2022},
NUMBER = {9},
PAGES = {2089--2099},
URL = {http://www.techscience.com/biocell/v46n9/47747},
ISSN = {1667-5746},
ABSTRACT = {The limited bioactivity of scaffold materials is an important factor that restricts the development of bone tissue engineering. Wnt3a activates the classic Wnt/β-catenin signaling pathway which effects bone growth and development by the accumulation of β-catenin in the nucleus. In this study, we fabricated 3D printed PCL scaffold with Wnt3a-induced murine bone marrow-derived stromal cell line ST2 decellularized matrix (Wnt3a-ST2-dCM-PCL) and ST2 decellularized matrix (ST2-dCM-PCL) by freeze-thaw cycle and DNase decellularization treatment which efficiently decellularized >90% DNA while preserved most protein. Compared to ST2-dCM-PCL, Wnt3a-ST2-dCM-PCL significantly enhanced newly-seeded ST2 proliferation, osteogenic differentiation and upregulated osteogenic marker genes alkaline phosphatase (<i>Alp</i>), <i>Runx2</i>, type I collagen (<i>Col 1</i>) and osteocalcin (<i>Ocn</i>) mRNA expression. After 14 days of osteogenic induction, Wnt3a-ST2-dCM-PCL promoted ST2 mineralization. These results demonstrated that Wnt3a-induced ST2 decellularized matrix improve scaffold materials’ osteoinductivity and osteoconductivity.},
DOI = {10.32604/biocell.2022.020069}
}