@Article{biocell.2022.020512,
AUTHOR = {ZHENKUI CUI, GUIQIN SUN, QUANZHI ZHAO},
TITLE = {Transcriptome analysis combined with metabolome analysis reveals the significant functions of <i>CesA</i> genes in cotton (<i>Gossypium hirsutum</i>) fiber length development},
JOURNAL = {BIOCELL},
VOLUME = {46},
YEAR = {2022},
NUMBER = {9},
PAGES = {2133--2144},
URL = {http://www.techscience.com/biocell/v46n9/47751},
ISSN = {1667-5746},
ABSTRACT = {Cotton is widely distributed worldwide, and improving the quality of its fiber is one of the most important tasks in cotton breeding. Cotton fibers are primarily composed of cellulose, which is synthesized by <i>CesA</i> complexes (CSCs). However, the functions of <i>CesA</i> genes in cotton fiber development have not been comprehensively analysed. In this study, the cotton transcriptome and metabolome were used to investigate the function o<i>f CesA</i> genes in fiber development. Finally, 321 metabolites were obtained, 84 of which were associated with the corresponding genes. Interestingly, a target gene named <i>Gh_A08G144300</i>, one of the <i>CesA</i> gene family members, was closely correlated with the development of cotton fibers. The target <i>CesA</i> gene <i>Gh_A08G144300</i> was analysed to determine its specific function in cotton fiber development. High-level gene expression of <i>Gh_A08G144300</i> was found at different fiber development stages by RNA-seq analysis, and the silencing of <i>Gh_A08G144300</i> visibly inhibited the growth of cotton fibers, showing that it is critical for their growth. This study provides an important reference for research on the gene function of <i>Gh_A08G144300</i> and the regulatory mechanism of fiber development in cotton.},
DOI = {10.32604/biocell.2022.020512}
}